1.1 Introduction The principal goal of the Experimental Animal Core is to house mice in the more cost effective and efficient manner in a central facility for all three of the proposed projects. The generation of transgenic mice has proven to be a very powerful tool in answering biologic questions, especially those directly relevant to human disease. However, animal modeling has become increasingly complex with many variables to consider including mouse strain, the selection of tissue specific promoters to modulate targeted gene expression, as well as overall expense. The function of Core B is to serve as a centralized resource to house and maintain the animals and to keep the overall costs of experimental animals to a minimum for the program. In addition. Dr. John Manis will act as a scientific resource to the investigators in planning for the generation of compound mutant mice with multiple genotypes for experimental analysis. Table 1 lists the various genetically engineered animals and normal control mouse strains that will be employed in the scientific studies of the program, housed in the core facility, and used during the course of this proposal. Each mouse strain will be housed and generated in direct proportion to the need ofthe investigator, with a breeding and management algorithm using a specialized software based method (Granite).

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Children's Hospital Boston
United States
Zip Code
Luo, Hongbo R (2014) A dual regulator of neutrophil recruitment. Blood 123:1983-5
Ueno, Shikiko; Lu, Jiayun; He, Jie et al. (2014) Aberrant expression of SALL4 in acute B cell lymphoblastic leukemia: mechanism, function, and implication for a potential novel therapeutic target. Exp Hematol 42:307-316.e8
Loison, Fabien; Zhu, Haiyan; Karatepe, Kutay et al. (2014) Proteinase 3-dependent caspase-3 cleavage modulates neutrophil death and inflammation. J Clin Invest 124:4445-58
Jo, Hakryul; Loison, Fabien; Luo, Hongbo R (2014) Microtubule dynamics regulates Akt signaling via dynactin p150. Cell Signal 26:1707-16
Gao, Chong; Dimitrov, Todor; Yong, Kol Jia et al. (2013) Targeting transcription factor SALL4 in acute myeloid leukemia by interrupting its interaction with an epigenetic complex. Blood 121:1413-21
Yong, Kol Jia; Gao, Chong; Lim, Joline S J et al. (2013) Oncofetal gene SALL4 in aggressive hepatocellular carcinoma. N Engl J Med 368:2266-76
Nombela-Arrieta, Cesar; Pivarnik, Gregory; Winkel, Beatrice et al. (2013) Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment. Nat Cell Biol 15:533-43
Li, Ailing; Yang, Youyang; Gao, Chong et al. (2013) A SALL4/MLL/HOXA9 pathway in murine and human myeloid leukemogenesis. J Clin Invest 123:4195-207
Gao, Chong; Kong, Nikki R; Li, Ailing et al. (2013) SALL4 is a key transcription regulator in normal human hematopoiesis. Transfusion 53:1037-49
Lu, Jiayun; Sun, Yan; Nombela-Arrieta, Cesar et al. (2012) Fak depletion in both hematopoietic and nonhematopoietic niche cells leadsýýtoýýhematopoietic stem cell expansion. Exp Hematol 40:307-17.e3

Showing the most recent 10 out of 15 publications