The Molecular Biology Core will provide expert and efficient laboratory services to Project 1 (Saper), Project 3 (Scammell), and Project 5 (Chamberiin). The major duties of Core B are management and genotyping of mouse colonies and performing in situ hybridization histochemistry. Core B will also produce new bacterial clones for in situ hybridization and new viral vectors as technology evolves. Core B is designed to optimize efficiency, accuracy, and expertise. The Core will manage large, shared mouse colonies to ensure a reliable and efficient supply of accurately genotyped mice for each Project. The Core also will centralize the performance of complex molecular techniques such as in situ hybridization histochemistry and bacterial culture so researchers in the Projects can focus on the science instead of debugging techniques Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL095491-01A1
Application #
7798791
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2010-03-01
Project End
2015-02-28
Budget Start
2010-03-01
Budget End
2011-02-28
Support Year
1
Fiscal Year
2010
Total Cost
$202,087
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
Scammell, Thomas E; Arrigoni, Elda; Lipton, Jonathan O (2017) Neural Circuitry of Wakefulness and Sleep. Neuron 93:747-765
Yang, Chun; McKenna, James T; Brown, Ritchie E (2017) Intrinsic membrane properties and cholinergic modulation of mouse basal forebrain glutamatergic neurons in vitro. Neuroscience 352:249-261
Landry, Shane A; Joosten, Simon A; Sands, Scott A et al. (2017) Response to a combination of oxygen and a hypnotic as treatment for obstructive sleep apnoea is predicted by a patient's therapeutic CPAP requirement. Respirology 22:1219-1224
Marques, Melania; Genta, Pedro R; Sands, Scott A et al. (2017) Effect of Sleeping Position on Upper Airway Patency in Obstructive Sleep Apnea Is Determined by the Pharyngeal Structure Causing Collapse. Sleep 40:
Azarbarzin, Ali; Sands, Scott A; Taranto-Montemurro, Luigi et al. (2017) Estimation of Pharyngeal Collapsibility During Sleep by Peak Inspiratory Airflow. Sleep 40:
Kroeger, Daniel; Ferrari, Loris L; Petit, Gaetan et al. (2017) Cholinergic, Glutamatergic, and GABAergic Neurons of the Pedunculopontine Tegmental Nucleus Have Distinct Effects on Sleep/Wake Behavior in Mice. J Neurosci 37:1352-1366
Rukhadze, Irma; Carballo, Nancy J; Bandaru, Sathyajit S et al. (2017) Catecholaminergic A1/C1 neurons contribute to the maintenance of upper airway muscle tone but may not participate in NREM sleep-related depression of these muscles. Respir Physiol Neurobiol 244:41-50
Kim, Bowon; Kocsis, Bernat; Hwang, Eunjin et al. (2017) Differential modulation of global and local neural oscillations in REM sleep by homeostatic sleep regulation. Proc Natl Acad Sci U S A 114:E1727-E1736
Kaur, Satvinder; Wang, Joshua L; Ferrari, Loris et al. (2017) A Genetically Defined Circuit for Arousal from Sleep during Hypercapnia. Neuron 96:1153-1167.e5
Geerling, Joel C; Yokota, Shigefumi; Rukhadze, Irma et al. (2017) K├Âlliker-Fuse GABAergic and glutamatergic neurons project to distinct targets. J Comp Neurol 525:1844-1860

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