The Electrophysiology Core will provide centralized services for electrophysiological studies, including preparation of in vitro brain slices, patch-clamp recordings, immunohistochemistry, and the associated image and data analysis. Project 3 (Scammell) and Project 5 (Chamberlin) will use this Core facility. Project 3 will use the services of the Core to examine how orexin and dynorphin increase forebrain activity. Specifically, these experiments will define the pre- and post-synaptic effects and the mechanism through which these neurotransmitters affect cortically-projecting neurons in the basal forebrain. We will also determine the peptide receptors and conductances involved in these responses. Project 5 will use the Electrophysiology Core to determine whether neurons of the Kolliker-Fuse (KF) nucleus are chemosensitive. We will determine which KF neurons are intrinsically responsive to changes of pH, and we will identify the mechanisms underlying these responses. These Projects will benefit from the expertise and many shared resources of the Core.

Public Health Relevance

The goal of the Electrophysiology Core is to provide resources and expertise for in vitro recordings for multiple investigators. The Projects will benefit from using a well-established in vitro electrophysiology laboratory. This centralized service will ensure consistent, high-quality recordings across Projects and will prevent duplication of equipment and resources.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095491-05
Application #
8634594
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
5
Fiscal Year
2014
Total Cost
$170,518
Indirect Cost
$72,519
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
Geerling, Joel C; Kim, Minjee; Mahoney, Carrie E et al. (2016) Genetic identity of thermosensory relay neurons in the lateral parabrachial nucleus. Am J Physiol Regul Integr Comp Physiol 310:R41-54
Abbott, Stephen B G; Machado, Natalia L S; Geerling, Joel C et al. (2016) Reciprocal Control of Drinking Behavior by Median Preoptic Neurons in Mice. J Neurosci 36:8228-37
Zant, Janneke C; Kim, Tae; Prokai, Laszlo et al. (2016) Cholinergic Neurons in the Basal Forebrain Promote Wakefulness by Actions on Neighboring Non-Cholinergic Neurons: An Opto-Dialysis Study. J Neurosci 36:2057-67
Taranto-Montemurro, Luigi; Edwards, Bradley A; Sands, Scott A et al. (2016) Desipramine Increases Genioglossus Activity and Reduces Upper Airway Collapsibility during Non-REM Sleep in Healthy Subjects. Am J Respir Crit Care Med 194:878-885
Genta, Pedro R; Edwards, Bradley A; Sands, Scott A et al. (2016) Tube Law of the Pharyngeal Airway in Sleeping Patients with Obstructive Sleep Apnea. Sleep 39:337-43
Arrigoni, Elda; Chen, Michael C; Fuller, Patrick M (2016) The anatomical, cellular and synaptic basis of motor atonia during rapid eye movement sleep. J Physiol 594:5391-414
Ferrari, L L; Agostinelli, L J; Krashes, M J et al. (2016) Dynorphin inhibits basal forebrain cholinergic neurons by pre- and postsynaptic mechanisms. J Physiol 594:1069-85
Vetrivelan, Ramalingam; Kong, Dong; Ferrari, Loris L et al. (2016) Melanin-concentrating hormone neurons specifically promote rapid eye movement sleep in mice. Neuroscience 336:102-113
Brown, Ritchie E; McKenna, James T (2015) Turning a Negative into a Positive: Ascending GABAergic Control of Cortical Activation and Arousal. Front Neurol 6:135
Sherman, David; Fuller, Patrick M; Marcus, Jacob et al. (2015) Anatomical Location of the Mesencephalic Locomotor Region and Its Possible Role in Locomotion, Posture, Cataplexy, and Parkinsonism. Front Neurol 6:140

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