The Electrophysiology Core will provide centralized services for electrophysiological studies, including preparation of in vitro brain slices, patch-clamp recordings, immunohistochemistry, and the associated image and data analysis. Project 3 (Scammell) and Project 5 (Chamberlin) will use this Core facility. Project 3 will use the services of the Core to examine how orexin and dynorphin increase forebrain activity. Specifically, these experiments will define the pre- and post-synaptic effects and the mechanism through which these neurotransmitters affect cortically-projecting neurons in the basal forebrain. We will also determine the peptide receptors and conductances involved in these responses. Project 5 will use the Electrophysiology Core to determine whether neurons of the Kolliker-Fuse (KF) nucleus are chemosensitive. We will determine which KF neurons are intrinsically responsive to changes of pH, and we will identify the mechanisms underlying these responses. These Projects will benefit from the expertise and many shared resources of the Core.
The goal of the Electrophysiology Core is to provide resources and expertise for in vitro recordings for multiple investigators. The Projects will benefit from using a well-established in vitro electrophysiology laboratory. This centralized service will ensure consistent, high-quality recordings across Projects and will prevent duplication of equipment and resources.
|Scammell, Thomas E; Arrigoni, Elda; Lipton, Jonathan O (2017) Neural Circuitry of Wakefulness and Sleep. Neuron 93:747-765|
|Yang, Chun; McKenna, James T; Brown, Ritchie E (2017) Intrinsic membrane properties and cholinergic modulation of mouse basal forebrain glutamatergic neurons in vitro. Neuroscience 352:249-261|
|Landry, Shane A; Joosten, Simon A; Sands, Scott A et al. (2017) Response to a combination of oxygen and a hypnotic as treatment for obstructive sleep apnoea is predicted by a patient's therapeutic CPAP requirement. Respirology 22:1219-1224|
|Marques, Melania; Genta, Pedro R; Sands, Scott A et al. (2017) Effect of Sleeping Position on Upper Airway Patency in Obstructive Sleep Apnea Is Determined by the Pharyngeal Structure Causing Collapse. Sleep 40:|
|Azarbarzin, Ali; Sands, Scott A; Taranto-Montemurro, Luigi et al. (2017) Estimation of Pharyngeal Collapsibility During Sleep by Peak Inspiratory Airflow. Sleep 40:|
|Kroeger, Daniel; Ferrari, Loris L; Petit, Gaetan et al. (2017) Cholinergic, Glutamatergic, and GABAergic Neurons of the Pedunculopontine Tegmental Nucleus Have Distinct Effects on Sleep/Wake Behavior in Mice. J Neurosci 37:1352-1366|
|Rukhadze, Irma; Carballo, Nancy J; Bandaru, Sathyajit S et al. (2017) Catecholaminergic A1/C1 neurons contribute to the maintenance of upper airway muscle tone but may not participate in NREM sleep-related depression of these muscles. Respir Physiol Neurobiol 244:41-50|
|Kim, Bowon; Kocsis, Bernat; Hwang, Eunjin et al. (2017) Differential modulation of global and local neural oscillations in REM sleep by homeostatic sleep regulation. Proc Natl Acad Sci U S A 114:E1727-E1736|
|Kaur, Satvinder; Wang, Joshua L; Ferrari, Loris et al. (2017) A Genetically Defined Circuit for Arousal from Sleep during Hypercapnia. Neuron 96:1153-1167.e5|
|Geerling, Joel C; Yokota, Shigefumi; Rukhadze, Irma et al. (2017) Kölliker-Fuse GABAergic and glutamatergic neurons project to distinct targets. J Comp Neurol 525:1844-1860|
Showing the most recent 10 out of 173 publications