The Analytical Core laboratory will provide biochemical and genotyping assay services as well as histology and immunohistochemical analyses for the individual projects within the Program Project. Biochemical assays that will be performed include ET-1, MCP-1, sICAM, IL-6, cGMP, nitrate/nitrite, microalbumin, tissue total protein, aldosterone, urinary albumin, para-aminohippuric acid, inulin, hematocrit, electrolytes creatinine, 8-isoprostane, thiobarbituric acid reactants, superoxide, measurements in urine, plasma, and/or tissue extracts from rats, mice, and humans. Techniques will include radioimmunoassay, ELISA, ion selective electrodes, and enzymatic assays, and binding assays. Genotyping assays will also be accomplished for the transgenic rats and mice that will be maintained by Core C. Histology and immunohistochemical analyses will provide hematoxylin &eosin (H&E), periodic acid-Schiff (PAS), Sirius red, Masson's blue trichrome, and specific immunohistochemical staining analyses for specific protein markers for the Program Project. The core will also provide resources for development of additional assays and/or histological and immunohistological analyses as needed by individual investigators. The core will assure assay and histology quality control through validation procedures.
The Program Project focuses on elucidating mechanisms by which the kidney controls sodium excretion, and therefore, has direct relevance to the serious health problem of salt-dependent hypertension and kidney disease. The Analytical Core plays an important role in conducting basic biochemical assays and histological analysis critical for the success of each of the participating projects.
| Kang, Kyu-Tae; Sullivan, Jennifer C; Pollock, Jennifer S (2018) Superoxide Dismutase Activity in Small Mesenteric Arteries Is Downregulated by Angiotensin II but Not by Hypertension. Toxicol Res 34:363-370 |
| De Miguel, Carmen; Sedaka, Randee; Kasztan, Malgorzata et al. (2018) Tauroursodeoxycholic acid (TUDCA) abolishes chronic high salt-induced renal injury and inflammation. Acta Physiol (Oxf) :e13227 |
| Johnston, Jermaine G; Pollock, David M (2018) Circadian regulation of renal function. Free Radic Biol Med 119:93-107 |
| Guan, Z; Wang, F; Cui, X et al. (2018) Mechanisms of sphingosine-1-phosphate-mediated vasoconstriction of rat afferent arterioles. Acta Physiol (Oxf) 222: |
| De Miguel, Carmen; Hamrick, William C; Hobbs, Janet L et al. (2017) Endothelin receptor-specific control of endoplasmic reticulum stress and apoptosis in the kidney. Sci Rep 7:43152 |
| Gohar, Eman Y; Kasztan, Malgorzata; Pollock, David M (2017) Interplay between renal endothelin and purinergic signaling systems. Am J Physiol Renal Physiol 313:F666-F668 |
| Gohar, Eman Y; Speed, Joshua S; Kasztan, Malgorzata et al. (2016) Activation of purinergic receptors (P2) in the renal medulla promotes endothelin-dependent natriuresis in male rats. Am J Physiol Renal Physiol 311:F260-7 |
| De Miguel, Carmen; Speed, Joshua S; Kasztan, Malgorzata et al. (2016) Endothelin-1 and the kidney: new perspectives and recent findings. Curr Opin Nephrol Hypertens 25:35-41 |
| Jin, Chunhua; Speed, Joshua S; Pollock, David M (2016) High salt intake increases endothelin B receptor function in the renal medulla of rats. Life Sci 159:144-147 |
| Gohar, Eman Y; Giachini, Fernanda R; Pollock, David M et al. (2016) Role of the endothelin system in sexual dimorphism in cardiovascular and renal diseases. Life Sci 159:20-29 |
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