The Lipid Mass Spectrometry Core is to provide support for all PPG Projects with state-of-the-art technology to quantify sphingosine-1-phosphate (SIP) and related signaling sphingolipids in tissues, biological fluids and endothelial cells. The task of reliable and sensitive quantitation of sphingolipid mediators and other small molecules derived from biological samples under normal and pathological conditions, in the presence of complex mixture of closely related compounds, is best resolved by tandem mass spectrometry in conjunction with liquid chromatography (LC/MS/MS). The Lipid Mass Spectrometry Core is equipped with AB Sciex 5500 Qtrap triple quadrupole-ion trap hybrid mass spectrometer and we have already developed reliable and sensitive electrospray ionization-tandem mass spectrometry-based methods for quntitation of major signaling sphingolipids such as SIP, ceramides and sphingoid bases as well as their synthetic analogs FTY720, its phosphate and phosphonate derivatives. Cellular and tissue samples generated within all 4 projects of the program will be centrally analyzed to provide uniformity and quality control to quantitative sphingolipid analyses within the Program Project. This approach will help integrate projects since SIP/ceramide changes are increasingly implicated in endotoxinand radiation-induced lung injury (Project 1), are potentially linked to the dynamic changes of SIP receptors during endotoxin- and radiation-induced lung injury (Project 2 and 4), are instrumental in mediating FTY720- phosphonate based therapies (Project 3), and might be biomarkers for the development of acute lung injury (Project 2) and radiation-induced pneumonitis (Project 4). SIP-ceramide levels can be manipulated via sphingosine kinases, SIP lyase (Project 1) and by FTY720P or its analogs (Project 3). The Mass Spectrometry Core is also integrated with other Core facilities and research programs of the University of Chicago with major emphasis on quantitation of lipid mediators involved in signal transduction, cardiovascular and pulmonary diseases. In summary. Mass Spectrometry Core will be central in providing data on signaling sphingolipidom changes as a result of acute lung injury and radiation-induced pneumonitis.

Public Health Relevance

Signaling sphingolipids are deeply involved in the development and resolution of Acute Lung Injury and radiation-induced pneumonitis. Lipid Mass Spectrometry Core will centralize the analyses of signaling sphingolipids and their synthetic analogs thus providing the uniformity of the analysis throughout the entire Program Project.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Illinois at Chicago
United States
Zip Code
Huang, Long Shuang; Natarajan, Viswanathan (2015) Sphingolipids in pulmonary fibrosis. Adv Biol Regul 57:55-63
Ni, Xiuqin; Epshtein, Yulia; Chen, Weiguo et al. (2014) Interaction of integrin ?4 with S1P receptors in S1P- and HGF-induced endothelial barrier enhancement. J Cell Biochem 115:1187-95
Wolfson, Rachel K; Mapes, Brandon; Garcia, Joe G N (2014) Excessive mechanical stress increases HMGB1 expression in human lung microvascular endothelial cells via STAT3. Microvasc Res 92:50-5
Chen, Jiwang; Tang, Haiyang; Sysol, Justin R et al. (2014) The sphingosine kinase 1/sphingosine-1-phosphate pathway in pulmonary arterial hypertension. Am J Respir Crit Care Med 190:1032-43
Testai, Fernando D; Kilkus, John P; Berdyshev, Evgeny et al. (2014) Multiple sphingolipid abnormalities following cerebral microendothelial hypoxia. J Neurochem 131:530-40
Usatyuk, Peter V; Fu, Panfeng; Mohan, Vijay et al. (2014) Role of c-Met/phosphatidylinositol 3-kinase (PI3k)/Akt signaling in hepatocyte growth factor (HGF)-mediated lamellipodia formation, reactive oxygen species (ROS) generation, and motility of lung endothelial cells. J Biol Chem 289:13476-91
Adyshev, Djanybek M; Elangovan, Venkateswaran Ramamoorthi; Moldobaeva, Nurgul et al. (2014) Mechanical stress induces pre-B-cell colony-enhancing factor/NAMPT expression via epigenetic regulation by miR-374a and miR-568 in human lung endothelium. Am J Respir Cell Mol Biol 50:409-18
Huang, Long Shuang; Mathew, Biji; Li, Haiquan et al. (2014) The mitochondrial cardiolipin remodeling enzyme lysocardiolipin acyltransferase is a novel target in pulmonary fibrosis. Am J Respir Crit Care Med 189:1402-15
Wang, Lichun; Sammani, Saad; Moreno-Vinasco, Liliana et al. (2014) FTY720 (s)-phosphonate preserves sphingosine 1-phosphate receptor 1 expression and exhibits superior barrier protection to FTY720 in acute lung injury. Crit Care Med 42:e189-99
Makarenko, Vladislav V; Usatyuk, Peter V; Yuan, Guoxiang et al. (2014) Intermittent hypoxia-induced endothelial barrier dysfunction requires ROS-dependent MAP kinase activation. Am J Physiol Cell Physiol 306:C745-52

Showing the most recent 10 out of 25 publications