The purpose of the Asthma Biorepository Core Tissue Processing and Cell Culture Core (Core C) component of this TPPG is to provide centralized expert handling of clinical samples obtained from human subjects by the Clinical Core. The tissue processing and cell culture activities of Core C will convert the clinical samples into materials (ainway cells, fiuid, slides, etc.) required for the research proposed in all four Projects. This core will also utilize explanted lungs to culture and provide human primary smooth muscle, fibroblast and epithelial cells to the projects in this TPPG. Furthermore, the Core will also work closely with Core D regarding the culturing of mouse smooth muscle cells and tracheal epithelial cells. There are many advantages to having these activities done by a single Core facility rather than in each investigator's own laboratory. Foremost, the personnel staffing of the Core are highly experienced in performing the required procedures in a meticulous and standardized manner. In addition Core C will be responsible for ensuring that samples are properiy characterized, inventoried, stored, and that data is maintained in a data base with appropriate access to TPPG investigators and other cores. In this capacity, the Core will allow for each clinical sample to be utilized to its full potential in multiple Projects. This Core will interact closely with all Cores in this TPPG to maintain the extensive database required to catalog and manage the processed clinical materials and generate coherent links among experimental results obtained with these materials.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
5P01HL103453-04
Application #
8686059
Study Section
Special Emphasis Panel (ZHL1)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
City
Cleveland
State
OH
Country
United States
Zip Code
Navaneethan, Udayakumar; Parsi, Mansour A; Lourdusamy, Vennisvasanth et al. (2015) Volatile organic compounds in bile for early diagnosis of cholangiocarcinoma in patients with primary sclerosing cholangitis: a pilot study. Gastrointest Endosc 81:943-9.e1
Cowan, Douglas C; Taylor, D Robin; Peterson, Laura E et al. (2015) Biomarker-based asthma phenotypes of corticosteroid response. J Allergy Clin Immunol 135:877-83.e1
Tang, W H Wilson; Wang, Zeneng; Shrestha, Kevin et al. (2015) Intestinal microbiota-dependent phosphatidylcholine metabolites, diastolic dysfunction, and adverse clinical outcomes in chronic systolic heart failure. J Card Fail 21:91-6
Alkhouri, N; Eng, K; Cikach, F et al. (2015) Breathprints of childhood obesity: changes in volatile organic compounds in obese children compared with lean controls. Pediatr Obes 10:23-9
Lauer, Mark E; Aytekin, Metin; Comhair, Suzy A et al. (2014) Modification of hyaluronan by heavy chains of inter-?-inhibitor in idiopathic pulmonary arterial hypertension. J Biol Chem 289:6791-8
Ratanamaneechat, Suphagaphan; Neumann, Donald R; Difilippo, Frank P et al. (2014) Redox imaging of inflammation in asthma. Am J Respir Crit Care Med 189:743-6
Kennedy, David J; Fan, Yiying; Wu, Yuping et al. (2014) Plasma ceruloplasmin, a regulator of nitric oxide activity, and incident cardiovascular risk in patients with CKD. Clin J Am Soc Nephrol 9:462-7
Tang, W H Wilson; Topol, Eric J; Fan, Yiying et al. (2014) Prognostic value of estimated functional capacity incremental to cardiac biomarkers in stable cardiac patients. J Am Heart Assoc 3:e000960
Navaneethan, Sankar D; Wehbe, Edgard; Heresi, Gustavo A et al. (2014) Presence and outcomes of kidney disease in patients with pulmonary hypertension. Clin J Am Soc Nephrol 9:855-63
Xu, Weiling; Janocha, Allison J; Leahy, Rachel A et al. (2014) A novel method for pulmonary research: assessment of bioenergetic function at the air-liquid interface. Redox Biol 2:513-9

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