Core C is designed as the Shared Resources Core. It will provide a number of centralized services to meet the key glycoscience analytical techniques required to achieve the aims set forth in each Project of this PEG. This core is central to the goals ofthe Program, offering several highly innovative technologies created by Program scientists specifically to interrogate glycan structure and function, and ensuring proper execution of a variety of technically-demanding procedures. The Core is divided into 4 components: (1) Glycoanalytic Component;(2) Adhesion Assay Component;(3) Ex Vivo Glycan Engineering Component;and (4) In Vitro Glycosyltransferase and Glycosidase Component. This core realizes the Program's intent to sustain an experimentally integrated effort, and ensures that data derived from glycoscience-specialized activities will be uniformly reproducible among all the Projects.

Public Health Relevance

Core C is designed as the Shared Resources Core. It will provide a number of centralized services to meet the key glycoscience analytical techniques required to achieve the aims set forth in each Project of this PEG.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL107146-01
Application #
8183660
Study Section
Special Emphasis Panel (ZHL1-CSR-H (F1))
Project Start
Project End
Budget Start
2011-07-01
Budget End
2012-05-31
Support Year
1
Fiscal Year
2011
Total Cost
$312,501
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115
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Liu, Gang; Cheng, Kai; Lo, Chi Y et al. (2017) A Comprehensive, Open-source Platform for Mass Spectrometry-based Glycoproteomics Data Analysis. Mol Cell Proteomics 16:2032-2047
Pachón-Peña, Gisela; Donnelly, Conor; Ruiz-Cañada, Catalina et al. (2017) A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells. Stem Cells 35:1080-1092
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Silva, Mariana; Fung, Ronald Kam Fai; Donnelly, Conor Brian et al. (2017) Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology. J Immunol 198:3576-3587
Buffone Jr, Alexander; Nasirikenari, Mehrab; Manhardt, Charles T et al. (2017) Leukocyte-borne ?(1,3)-fucose is a negative regulator of ?2-integrin-dependent recruitment in lung inflammation. J Leukoc Biol 101:459-470
Sackstein, Robert; Schatton, Tobias; Barthel, Steven R (2017) T-lymphocyte homing: an underappreciated yet critical hurdle for successful cancer immunotherapy. Lab Invest 97:669-697
Cheng, Kai; Zhou, Yusen; Neelamegham, Sriram (2017) DrawGlycan-SNFG: a robust tool to render glycans and glycopeptides with fragmentation information. Glycobiology 27:200-205
Dougher, Christopher W L; Buffone Jr, Alexander; Nemeth, Michael J et al. (2017) The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis. J Leukoc Biol 102:507-516
Lee, Jungmin; Dykstra, Brad; Spencer, Joel A et al. (2017) mRNA-mediated glycoengineering ameliorates deficient homing of human stem cell-derived hematopoietic progenitors. J Clin Invest 127:2433-2437

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