Abstract

Core C is designed as the Shared Resources Core. It will provide a number of centralized services to meet the key glycoscience analytical techniques required to achieve the aims set forth in each Project of this PEG. This core is central to the goals ofthe Program, offering several highly innovative technologies created by Program scientists specifically to interrogate glycan structure and function, and ensuring proper execution of a variety of technically-demanding procedures. The Core is divided into 4 components: (1) Glycoanalytic Component;(2) Adhesion Assay Component;(3) Ex Vivo Glycan Engineering Component;and (4) In Vitro Glycosyltransferase and Glycosidase Component. This core realizes the Program's intent to sustain an experimentally integrated effort, and ensures that data derived from glycoscience-specialized activities will be uniformly reproducible among all the Projects.

Public Health Relevance

Core C is designed as the Shared Resources Core. It will provide a number of centralized services to meet the key glycoscience analytical techniques required to achieve the aims set forth in each Project of this PEG.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL107146-03
Application #
8477252
Study Section
Special Emphasis Panel (ZHL1-CSR-H)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
3
Fiscal Year
2013
Total Cost
$269,744
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Mondal, Nandini; Dykstra, Brad; Lee, Jungmin et al. (2018) Distinct human ?(1,3)-fucosyltransferases drive Lewis-X/sialyl Lewis-X assembly in human cells. J Biol Chem 293:7300-7314
Donnelly, Conor; Dykstra, Brad; Mondal, Nandini et al. (2018) Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression. Sci Rep 8:420
Carrascal, Mylène A; Silva, Mariana; Ramalho, José S et al. (2018) Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation. Mol Oncol 12:579-593
Irons, Eric E; Lau, Joseph T Y (2018) Systemic ST6Gal-1 Is a Pro-survival Factor for Murine Transitional B Cells. Front Immunol 9:2150
Videira, Paula A; Silva, Mariana; Martin, Kyle C et al. (2018) Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration. J Immunol 201:1030-1043
Carrascal, Mylène A; Talina, Catarina; Borralho, Paula et al. (2018) Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue. BMC Cancer 18:495
Silva, Mariana; Videira, Paula A; Sackstein, Robert (2017) E-Selectin Ligands in the Human Mononuclear Phagocyte System: Implications for Infection, Inflammation, and Immunotherapy. Front Immunol 8:1878
Manhardt, Charles T; Punch, Patrick R; Dougher, Christopher W L et al. (2017) Extrinsic sialylation is dynamically regulated by systemic triggers in vivo. J Biol Chem 292:13514-13520
Sackstein, Robert; Schatton, Tobias; Barthel, Steven R (2017) T-lymphocyte homing: an underappreciated yet critical hurdle for successful cancer immunotherapy. Lab Invest 97:669-697
Silva, Mariana; Fung, Ronald Kam Fai; Donnelly, Conor Brian et al. (2017) Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology. J Immunol 198:3576-3587

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