All projects will be supported by our High-Resolution Optical Imaging Core A (Boas/Sakadzic). This core will support the theme of functional coupling between glial, endothelial, and neuronal cells by using both in vivo and in vitro novel optical imaging to dissect the mechanisms of brain plasticity after cerebral ischemia . Novel optical imaging technologies have unique capability to measure the dynamics of glia, endothelium, and neurons as well as hemodynamic, metabolic, and electrophysiologic responses upon cerebral injury at the extensive range of temporal and spatial scales. Subcellular structures such as glial processes and neuronal spines will be assessed both in vivo and in vitro by Two-Photon Microscopy (TPM), and very fine subcellular interactions will be imaged by sub-diffraction-limit Stohastic Optical Reconstruction Microscopy (STORM). Optical Coherence Tomography (OCT) is unique in its ability to assess large cortical area and depth with high-resolution through a thin skull and it will be used to image longitudinally the recovery and remodeling of blood flow and vascular morphology after stroke in mice. These studies will be complemented by advanced intravascular and tissue oxygenation measurements by two-photon phosphorescence lifetime microscopy. Our core will also support the optogenetics studies of glial activation as well as the Optical Coherence Microscopy (OCM) - based whole brain imaging of cellular morphology and tractography. Various standard imaging technologies such as TPM imaging of calcium dynamics, Voltage-Sensitive Dye Imaging (VSDI), and multispectral and speckle imaging of hemodynamics will be supported too. All imaging procedures will be accompanied by the advanced data processing and modeling algorithms developed by our core. Investigators in projects 1-3 have a track record of productive collaborations with our core that resulted in numerous advances in the field and a multitude of joint publications over the last decade. This core will directly interact with all 3 projects to utilize advanced optical methods to study the coupling between glial, endothelial, and neuronal cells in response to ischemia.

Public Health Relevance

Cell-cell signaling in the neurovascular unit may underlie mechanisms of recovery after stroke and brain injury. This Core will provide high resolution optical imaging methods to investigate these mechanisms in all cell and animal models across all projects.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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Massachusetts General Hospital
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Rasmussen, Peter M; Smith, Amy F; Sakadži?, Sava et al. (2017) Model-based inference from microvascular measurements: Combining experimental measurements and model predictions using a Bayesian probabilistic approach. Microcirculation 24:
Kisler, Kassandra; Nelson, Amy R; Rege, Sanket V et al. (2017) Pericyte degeneration leads to neurovascular uncoupling and limits oxygen supply to brain. Nat Neurosci 20:406-416
Yaseen, Mohammad A; Sutin, Jason; Wu, Weicheng et al. (2017) Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo. Biomed Opt Express 8:2368-2385
Tang, Jianbo; Erdener, Sefik Evren; Li, Baoqiang et al. (2017) Shear-induced diffusion of red blood cells measured with dynamic light scattering-optical coherence tomography. J Biophotonics :
Egawa, Naohiro; Lok, Josephine; Washida, Kazuo et al. (2017) Mechanisms of Axonal Damage and Repair after Central Nervous System Injury. Transl Stroke Res 8:14-21
Xing, Changhong; Lo, Eng H (2017) Help-me signaling: Non-cell autonomous mechanisms of neuroprotection and neurorecovery. Prog Neurobiol 152:181-199
Pouliot, Philippe; Gagnon, Louis; Lam, Tina et al. (2017) Magnetic resonance fingerprinting based on realistic vasculature in mice. Neuroimage 149:436-445
Wang, Hui; Magnain, Caroline; Sakadži?, Sava et al. (2017) Characterizing the optical properties of human brain tissue with high numerical aperture optical coherence tomography. Biomed Opt Express 8:5617-5636
Mandeville, Emiri T; Ayata, Cenk; Zheng, Yi et al. (2017) Translational MR Neuroimaging of Stroke and Recovery. Transl Stroke Res 8:22-32
Xu, Kui; Boas, David A; Sakadži?, Sava et al. (2017) Brain Tissue PO2 Measurement During Normoxia and Hypoxia Using Two-Photon Phosphorescence Lifetime Microscopy. Adv Exp Med Biol 977:149-153

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