CORE D - Histology and Image Analysis Core - Theresa Connors - PI The goal of the Histology and Image Analysis Core is to identify and quantify the anatomical substrates of functional outcomes tested in the Electrophysiological and Behavior Cores and in the individual projects. This core has evolved over 20+ years to insure standardized throughput of the large quantity of specimens generated by individual projects of the PPG. We have found that training staff from each project and providing a well organized and stocked facility where work can be performed with guidance from core staff is the most efficient way to utilize resources. The Histology and Image Analysis Core maintains a database of all tissue processing, histological and immunohistochemical (IHC) procedures used by PPG personnel. In addition, the core staff oversees training on and maintenance of our image acquisition and analysis systems. The core director and technician are skilled in working with a wide variety of antibodies and procedures used to identify neurotransmitters and neuromodulators, cytoskeleton components, extracellular matrix and adhesion molecules, growth factors, and non-neuronal cells as well as fiuorescence and DAB labeling techniques. Core personnel are also well versed in the operation and application of the image analysis microscopes and softiA/are. The core strives to maintain quality control of materials and procedures used in all PPG projects by: requiring training prior to using core resources, providing stocks of all routinely used buffers and solutions, regularly updating the database of antibodies and procedures and providing guidance in the planning and execution of projects in the core lab. Testing and implementation of new techniques is an essential Core function. This has increased the efficiency of the PPG Histology and Image Analysis Core, reducing start-up time for new procedures while also reducing duplication and waste. With centralized training, equipment, supplies and a database for methods, the Histology and Image Analysis Core has evolved as the model for other Core Labs in the Program Proiect

Public Health Relevance

The core works to develop new methods for anatomical study of the effects of innovative therapies promoting repair, recovery and regeneration after SCI. Extensive experience of Pis and core personnel enable state of the art analysis crucial to the ongoing progress of this PPG. Project investigators with expertise in other fields can take advantage of anatomical analyses that may not be available in their labs.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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Drexel University
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Twiss, Jeffery L; Fainzilber, Mike (2016) Neuroproteomics: How Many Angels can be Identified in an Extract from the Head of a Pin? Mol Cell Proteomics 15:341-3
Detloff, Megan Ryan; Quiros-Molina, Daniel; Javia, Amy S et al. (2016) Delayed Exercise Is Ineffective at Reversing Aberrant Nociceptive Afferent Plasticity or Neuropathic Pain After Spinal Cord Injury in Rats. Neurorehabil Neural Repair 30:685-700
Sachdeva, Rahul; Farrell, Kaitlin; McMullen, Mary-Katharine et al. (2016) Dynamic Changes in Local Protein Synthetic Machinery in Regenerating Central Nervous System Axons after Spinal Cord Injury. Neural Plast 2016:4087254
Jin, Y; Bouyer, J; Shumsky, J S et al. (2016) Transplantation of neural progenitor cells in chronic spinal cord injury. Neuroscience 320:69-82
Sachdeva, Rahul; Theisen, Catherine C; Ninan, Vinu et al. (2016) Exercise dependent increase in axon regeneration into peripheral nerve grafts by propriospinal but not sensory neurons after spinal cord injury is associated with modulation of regeneration-associated genes. Exp Neurol 276:72-82
Yuan, Xiao-bing; Jin, Ying; Haas, Christopher et al. (2016) Guiding migration of transplanted glial progenitor cells in the injured spinal cord. Sci Rep 6:22576
Twiss, Jeffery L; Kalinski, Ashley L; Sachdeva, Rahul et al. (2016) Intra-axonal protein synthesis - a new target for neural repair? Neural Regen Res 11:1365-1367
Hayakawa, Kazuo; Haas, Christopher; Fischer, Itzhak (2016) Examining the properties and therapeutic potential of glial restricted precursors in spinal cord injury. Neural Regen Res 11:529-33
Jin, Ying; Bouyer, Julien; Haas, Christopher et al. (2015) Evaluation of the anatomical and functional consequences of repetitive mild cervical contusion using a model of spinal concussion. Exp Neurol 271:175-88
Hayakawa, Kazuo; Haas, Christopher; Jin, Ying et al. (2015) Glial restricted precursors maintain their permissive properties after long-term expansion but not following exposure to pro-inflammatory factors. Brain Res 1629:113-25

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