CORE F - Electrophysiology - Marie-Pascale Cote, Ph.D. - PI Molecular and histology techniques are routinely used to assess if therapeutic interventions successfully enhance axonal regeneration and impact spinal plasticity after SCI. A limitation ofthese techniques relies in their inability to confirm an effective translation ofthese changes in functional modulation (i.e. conduction in specific pathways and/or influence netiA/ork activity). Regenerated axons that have extended beyond a certain point does not necessarily imply that they form synapses, much less that they are effective and functional. Although behavioral testing addresses part of this issue, inter-individual variability can introduce complexity in the interpretation of data and does not give details about which cell/network is responsible for recovered function in sensorimotor systems. The goal of the Electrophysiological Core is to provide more sophisticated and detailed testing of function in subsets of experimental animals and more specifically to provide assistance to laboratories that do not have the expertise/equipment to carry out this type of experiment The electrophyhsiological core offers assistance in experimental design and oversees training of staff from individual projects that is tailored to their individual needs. The core assures unformity of procedures for a given testing paradigm and access to physiological testing and methods across projects. The core also develops and maintains useful test apparatus, procedures and software for analysis. The primary focus of this Core is to train individuals to evaluate the efficacy of interventions after SCI using the appropriate electrophysiological methods. The Director will assist the Principal Investigator in determining a suitable experimental design and train Project personnel to perform this test Training will include the operation of devices required for a given protocol as well as performing the procedure, collecting data and analyzing data. The Core also offers assistance to interpret data as needed.

Public Health Relevance

Molecular/histology techniques can effectively confirm axon regeneration through transplants, but are limited in their ability to address if axons are conducting and if newly formed synapses are function. This Core will provide guidance and training in order to address these criticial questions and provide assistance to laboratories that do not have the expertise/equipment to carry out this type of experiment

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
Project #
Application #
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Drexel University
United States
Zip Code
Twiss, Jeffery L; Fainzilber, Mike (2016) Neuroproteomics: How Many Angels can be Identified in an Extract from the Head of a Pin? Mol Cell Proteomics 15:341-3
Detloff, Megan Ryan; Quiros-Molina, Daniel; Javia, Amy S et al. (2016) Delayed Exercise Is Ineffective at Reversing Aberrant Nociceptive Afferent Plasticity or Neuropathic Pain After Spinal Cord Injury in Rats. Neurorehabil Neural Repair 30:685-700
Sachdeva, Rahul; Farrell, Kaitlin; McMullen, Mary-Katharine et al. (2016) Dynamic Changes in Local Protein Synthetic Machinery in Regenerating Central Nervous System Axons after Spinal Cord Injury. Neural Plast 2016:4087254
Jin, Y; Bouyer, J; Shumsky, J S et al. (2016) Transplantation of neural progenitor cells in chronic spinal cord injury. Neuroscience 320:69-82
Sachdeva, Rahul; Theisen, Catherine C; Ninan, Vinu et al. (2016) Exercise dependent increase in axon regeneration into peripheral nerve grafts by propriospinal but not sensory neurons after spinal cord injury is associated with modulation of regeneration-associated genes. Exp Neurol 276:72-82
Yuan, Xiao-bing; Jin, Ying; Haas, Christopher et al. (2016) Guiding migration of transplanted glial progenitor cells in the injured spinal cord. Sci Rep 6:22576
Twiss, Jeffery L; Kalinski, Ashley L; Sachdeva, Rahul et al. (2016) Intra-axonal protein synthesis - a new target for neural repair? Neural Regen Res 11:1365-1367
Hayakawa, Kazuo; Haas, Christopher; Fischer, Itzhak (2016) Examining the properties and therapeutic potential of glial restricted precursors in spinal cord injury. Neural Regen Res 11:529-33
Jin, Ying; Bouyer, Julien; Haas, Christopher et al. (2015) Evaluation of the anatomical and functional consequences of repetitive mild cervical contusion using a model of spinal concussion. Exp Neurol 271:175-88
Hayakawa, Kazuo; Haas, Christopher; Jin, Ying et al. (2015) Glial restricted precursors maintain their permissive properties after long-term expansion but not following exposure to pro-inflammatory factors. Brain Res 1629:113-25

Showing the most recent 10 out of 46 publications