Summary for Project 3: During the previous grant period we made the unexpected finding that spontaneous release of vesicles (miniature endplate currents, MEPCs) plays an important role in regulating the number of releasable vesicles (n) at the mouse neuromuscular junction (NMJ) in vivo. During preliminary studies we found that reducing the binding of acetylcholine to acetylcholine receptors (AChRs) during MEPCs in an ex vivo NMJ preparation triggers an increase in n within minutes. Our preliminary data suggests this is due to alteration of a retrograde signal initiated by opening of AChRs on muscle. Taken together our findings suggest there is a continuous retrograde signal from muscle that operates on a minute by minute time scale to adjust presynaptic function of the motoneuron. Our use of an ex vivo preparation allows for detailed study of this ongoing signaling that is not possible in vivo. In this proposal we will use both in vivo and ex vivo studies to dissect the molecular signaling underlying this pathway. A better understanding of this pathway will have implications for understanding synaptic plasticity and the response of motoneurons to injury including changes in excitability and loss of synaptic inputs. We hypothesize that disruption of the trophic signaling pathway we are studying at the NMJ is a trigger that induces potentially maladaptive spinal cord plasticity following peripheral nerve injury. Our findings could have implications for the motoneuron disease spinal muscular atrophy as well as the neuromuscular disease myasthenia gravis.

Public Health Relevance

Our work has implications for spinal cord function following peripheral nerve injury, the motoneuron disease spinal muscular atrophy as well as the neuromuscular disease myasthenia gravis. Our work also has basic science implications as it informs us about a signaling pathway that may play a role in synaptic plasticity at a number of synapses in the mammalian nervous system.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS057228-07
Application #
8627654
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
7
Fiscal Year
2014
Total Cost
$430,797
Indirect Cost
$135,732
Name
Wright State University
Department
Type
DUNS #
047814256
City
Dayton
State
OH
Country
United States
Zip Code
45435
Obeidat, Ahmed Z; Nardelli, Paul; Powers, Randall K et al. (2014) Modulation of motoneuron firing by recurrent inhibition in the adult rat in vivo. J Neurophysiol 112:2302-15
Rotterman, Travis M; Nardelli, Paul; Cope, Timothy C et al. (2014) Normal distribution of VGLUT1 synapses on spinal motoneuron dendrites and their reorganization after nerve injury. J Neurosci 34:3475-92
Deardorff, Adam S; Romer, Shannon H; Sonner, Patrick M et al. (2014) Swimming against the tide: investigations of the C-bouton synapse. Front Neural Circuits 8:106
Romer, Shannon H; Dominguez, Kathleen M; Gelpi, Marc W et al. (2014) Redistribution of Kv2.1 ion channels on spinal motoneurons following peripheral nerve injury. Brain Res 1547:1-15
Zhang, Jingming; Lanuza, Guillermo M; Britz, Olivier et al. (2014) V1 and v2b interneurons secure the alternating flexor-extensor motor activity mice require for limbed locomotion. Neuron 82:138-50
Koesters, Andrew; Engisch, Kathrin L; Rich, Mark M (2014) Decreased cardiac excitability secondary to reduction of sodium current may be a significant contributor to reduced contractility in a rat model of sepsis. Crit Care 18:R54
Deardorff, Adam S; Romer, Shannon H; Deng, Zhihui et al. (2013) Expression of postsynaptic Ca2+-activated K+ (SK) channels at C-bouton synapses in mammalian lumbar -motoneurons. J Physiol 591:875-97
Nardelli, Paul; Khan, Jaffar; Powers, Randall et al. (2013) Reduced motoneuron excitability in a rat model of sepsis. J Neurophysiol 109:1775-81
Wang, Xueyong; Wang, Qingbo; Yang, Shuzhang et al. (2011) Impaired activity-dependent plasticity of quantal amplitude at the neuromuscular junction of Rab3A deletion and Rab3A earlybird mutant mice. J Neurosci 31:3580-8
Bullinger, Katie L; Nardelli, Paul; Pinter, Martin J et al. (2011) Permanent central synaptic disconnection of proprioceptors after nerve injury and regeneration. II. Loss of functional connectivity with motoneurons. J Neurophysiol 106:2471-85

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