The long-term goal ofthe project is to identify common and distinct features of behavioral changes after three types of brain injury (subarachnoid hemorhage, intracerebral hemorhage, and traumatic brain injury) and the response to therapeutic interventions (osteopontin, anti-PDGF, Cav-AP) that protect the blood brain barrier and reduce brain swelling. The Behavioral Core will complement the scientific role ofthe project by implementing methods that can directly compare and contrast injury phenotype and treatment outcomes on a large number of subjects. The Behavioral Core has three basic goals for this project: ? Acquire data encompassing a broad range of behavioral domains (motor, learning, short-term memory, long-term memory, anxiety, depression, balance / coordination) over 4 time points (baseline, 24 hours postinjury, 72 hours post-injury, and 25-35 days post-injury). * Determine the effects of injury type (control vs. SAH vs. ICH vs. TBI), treatment (none vs. osteopontin vs. anti-PDGF vs. Cav-AP) and time (e.g., days 1 and 3, days 25-35) on the overall behavioral profile, as well as injury x treatment x time interactions. * Determine relationships between behavior and biomarkers from each model using data from the other Cores. Statistical modeling will allow us to determine which behavioral deficits tend to cluster together, suggesting common biological mechanisms. Data will be collected and analyzed in waves, which will allow for constant quality control and cost effecfiveness. For example, if it becomes clear that a particular test is ineffective in determining group difference, that test may be removed from the battery to save time and money.

Public Health Relevance

Behavior represents the final common pathway of neurological disorders. Identification of common and distinct features of brain injuries and treatments will help to determine the relationships between various behaviors and different brain areas, as well as the optimal treatment strategy and dosage.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Program Projects (P01)
Project #
Application #
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Loma Linda University
Loma Linda
United States
Zip Code
Zhan, Yan; Krafft, Paul R; Lekic, Tim et al. (2015) Imatinib preserves blood-brain barrier integrity following experimental subarachnoid hemorrhage in rats. J Neurosci Res 93:94-103
Zheng, Yun; Hu, Qin; Manaenko, Anatol et al. (2015) 17?-Estradiol attenuates hematoma expansion through estrogen receptor ?/silent information regulator 1/nuclear factor-kappa b pathway in hyperglycemic intracerebral hemorrhage mice. Stroke 46:485-91
Liu, Fei; Hu, Qin; Li, Bo et al. (2014) Recombinant milk fat globule-EGF factor-8 reduces oxidative stress via integrin ?3/nuclear factor erythroid 2-related factor 2/heme oxygenase pathway in subarachnoid hemorrhage rats. Stroke 45:3691-7
Chen, Sheng; Feng, Hua; Sherchan, Prativa et al. (2014) Controversies and evolving new mechanisms in subarachnoid hemorrhage. Prog Neurobiol 115:64-91
Fujii, Mutsumi; Sherchan, Prativa; Soejima, Yoshiteru et al. (2014) Cannabinoid receptor type 2 agonist attenuates apoptosis by activation of phosphorylated CREB-Bcl-2 pathway after subarachnoid hemorrhage in rats. Exp Neurol 261:396-403
Krafft, Paul R; McBride, Devin W; Lekic, Tim et al. (2014) Correlation between subacute sensorimotor deficits and brain edema in two mouse models of intracerebral hemorrhage. Behav Brain Res 264:151-60
Ma, Qingyi; Chen, Sheng; Hu, Qin et al. (2014) Reply: To PMID 24273204. Ann Neurol 75:972-3
Li, Qian; Khatibi, Nikan; Zhang, John H (2014) Vascular neural network: the importance of vein drainage in stroke. Transl Stroke Res 5:163-6
Altay, Orhan; Suzuki, Hidenori; Hasegawa, Yu et al. (2014) Isoflurane on brain inflammation. Neurobiol Dis 62:365-71
Fujii, Mutsumi; Sherchan, Prativa; Krafft, Paul R et al. (2014) Cannabinoid type 2 receptor stimulation attenuates brain edema by reducing cerebral leukocyte infiltration following subarachnoid hemorrhage in rats. J Neurol Sci 342:101-6

Showing the most recent 10 out of 11 publications