Abstract

While methods to image fluorescent molecules have advanced dramatically in recent years, the resolution of electron microscopy remains unsurpassed. In the state of Kentucky, there are numerous research programs that require the resolution that can only be achieved by the interaction of a beam of electrons with a specimen to distinguish ultrastructural features of tissues, cell components, viruses or bacteria. However, due to declining institutional support for electron microscopy facilities, many established Kentucky investigators have utilized facilities and expertise outside the state for their electron microscopic analyses. Furthermore, new Kentucky investigators are hesitant to add electron microscopy to their research programs without accessible training and technical support. We propose to address these gaps in infrastructure and education by establishing a statewide electron microscopy core to facilitate equipment sharing, provide technical support, and offer training.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
2P20GM103436-19
Application #
9673305
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2019-05-01
Budget End
2020-04-30
Support Year
19
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
University of Louisville
Department
Type
DUNS #
057588857
City
Louisville
State
KY
Country
United States
Zip Code
40292

  Publications

Masterson, S P; Zhou, N; Akers, B K et al. (2018) Ultrastructural and optogenetic dissection of V1 corticotectal terminal synaptic properties. J Comp Neurol :
Monroe, Jerry D; Millay, Matthew H; Patty, Blaine G et al. (2018) The curcuminoid, EF-24, reduces cisplatin-mediated reactive oxygen species in zebrafish inner ear auditory and vestibular tissues. J Clin Neurosci 57:152-156
Brausch, Amy M; Woods, Sherry E (2018) Emotion Regulation Deficits and Nonsuicidal Self-Injury Prospectively Predict Suicide Ideation in Adolescents. Suicide Life Threat Behav :
Colter, Breann T; Garber, Helen Frances; Fleming, Sheila M et al. (2018) Ahr and Cyp1a2 genotypes both affect susceptibility to motor deficits following gestational and lactational exposure to polychlorinated biphenyls. Neurotoxicology 65:125-134
Marczinski, Cecile A; Stamates, Amy L; Maloney, Sarah F (2018) Differential development of acute tolerance may explain heightened rates of impaired driving after consumption of alcohol mixed with energy drinks versus alcohol alone. Exp Clin Psychopharmacol 26:147-155
Lee, Juyeun; Park, Nogi; Park, Joo Youn et al. (2018) Induction of Immunosuppressive CD8+CD25+FOXP3+ Regulatory T Cells by Suboptimal Stimulation with Staphylococcal Enterotoxin C1. J Immunol 200:669-680
King, Rodney A; Perez, Meghan T (2018) Sequence Analysis of a Hybrid HK022/? Bacteriophage and the Precise Identification of the ?b515 and ?b519 Deletion Endpoints. Microbiol Resour Announc 7:
Gonzalez, O A; Kirakodu, S; Novak, M J et al. (2018) Comparative analysis of microbial sensing molecules in mucosal tissues with aging. Immunobiology 223:279-287
Ha, Dat Thinh; Ghosh, Sthitodhi; Ahn, Chong H et al. (2018) Pathogen-specific DNA sensing with engineered zinc finger proteins immobilized on a polymer chip. Analyst 143:4009-4016
Song, Ming; Li, Xiaohong; Zhang, Xiang et al. (2018) Dietary copper-fructose interactions alter gut microbial activity in male rats. Am J Physiol Gastrointest Liver Physiol 314:G119-G130

Showing the most recent 10 out of 193 publications