The Animal Models and Phenotyping Core (Core D) has four major goals. 1) Its primary function is to support the research projects of COBRE-supported Junior Investigators by providing state-of-the-art facilities and intellectual capability for the development and characterization of new and existing animal models used in diabetes and obesity research. Other goals are: 2) to provide training and education to Diabetes and Obesity Center junior investigators and members;3) to develop new models, techniques and protocols to further diabetes and obesity research;and, 4) to become a self-sustaining entity that supports the Diabetes and Obesity Center. While the Animal Core will preferentially address the needs of the COBRE-supported junior investigators, it will also assist in the development of new protocols, maintenance of unique mouse lines and creation of new models for other Diabetes and Obesity Center investigators. It will provide a comprehensive assessment of animal adiposity, glucose, insulin and pyruvate tolerance, physical activity, body temperature, food and water intake, oxygen consumption and carbon dioxide production in order to evaluate the overall metabolic condition ofthe model animal under environmental challenges including high fat diet. Training and educating investigators is an integral function of the Animal Core and it will continue to provide education to Center junior investigators and members. The Animal Core has trained personnel who in turn educate individual junior investigators on how to perform metabolic phenotyping tests correctly. The Animal Core has and will continue to develop new models of interest to investigators for specific mechanistic purposes. It will expand its operations to support future needs by adding infrastructure, equipment and by developing state-of-the-art animal models and by establishing new methods, refining assays and using statistical modeling approaches useful in metabolic phenotyping. The Animal Core intends to become a selfsustaining entity that will promote the long-term stability of the Center by providing essential services and developing new and unique capabilities for individual and multi-PI projects.

Public Health Relevance

The metabolic phenotyping of animal models used in diabetes and obesity research is an integral component of all COBRE-supported Junior Investigator-initated projects and for members ofthe Diabetes and Obesity Center. As such, Core D provides the infrastructure, animal models, techniques and oversight to support the in-depth and state-of-the-art characterization of metabolic phenotypes in models of obesity and diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103492-07
Application #
8711508
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
7
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Louisville
Department
Type
DUNS #
City
Louisville
State
KY
Country
United States
Zip Code
40202
Nystoriak, Matthew A; Bhatnagar, Aruni (2018) Cardiovascular Effects and Benefits of Exercise. Front Cardiovasc Med 5:135
Nystoriak, Matthew A; Navedo, Manuel F (2018) Regulation of microvascular function by voltage-gated potassium channels: New tricks for an ""ancient"" dog. Microcirculation 25:
Haberzettl, Petra; Conklin, Daniel J; Abplanalp, Wesley T et al. (2018) Inhalation of Fine Particulate Matter Impairs Endothelial Progenitor Cell Function Via Pulmonary Oxidative Stress. Arterioscler Thromb Vasc Biol 38:131-142
Mehra, Parul; Guo, Yiru; Nong, Yibing et al. (2018) Cardiac mesenchymal cells from diabetic mice are ineffective for cell therapy-mediated myocardial repair. Basic Res Cardiol 113:46
Ghosh Dastidar, Shubha; Jagatheesan, Ganapathy; Haberzettl, Petra et al. (2018) Glutathione S-transferase P Deficiency Induces Glucose Intolerance via JNK-dependent Enhancement of Hepatic Gluconeogenesis. Am J Physiol Endocrinol Metab :
Baba, Shahid P; Zhang, Deqing; Singh, Mahavir et al. (2018) Deficiency of aldose reductase exacerbates early pressure overload-induced cardiac dysfunction and autophagy in mice. J Mol Cell Cardiol 118:183-192
Hosen, Mohammed Rabiul; Militello, Giuseppe; Weirick, Tyler et al. (2018) Airn Regulates Igf2bp2 Translation in Cardiomyocytes. Circ Res 122:1347-1353
Dassanayaka, Sujith; Zheng, Yuting; Gibb, Andrew A et al. (2018) Cardiac-specific overexpression of aldehyde dehydrogenase 2 exacerbates cardiac remodeling in response to pressure overload. Redox Biol 17:440-449
Dwenger, Marc M; Ohanyan, Vahagn; Navedo, Manuel F et al. (2018) Coronary microvascular Kv1 channels as regulatory sensors of intracellular pyridine nucleotide redox potential. Microcirculation 25:
Jin, Lexiao; Lipinski, Alexandra; Conklin, Daniel J (2018) A Simple Method for Normalization of Aortic Contractility. J Vasc Res 55:177-186

Showing the most recent 10 out of 110 publications