Temperature control of the C. elegans circadian clock Daily (circadian) rhythms control multiple aspects of human behavior and physiology (e.g. sleep, body temperature) and disruption of these rhythms can either cause or affect the severity of most neurological disorders such as stroke and Alzheimer's disease. These circadian rhythms are driven by clocks in our brain and body that can be entrained by daily light and/or temperature cycles. In depth analyses have identified the physiological mechanisms comprising these light-entrained clocks in humans and most model organisms studied, but how temperature information controls these clocks is unclear. Our research has established the nematode Caenorhabditis elegans as a powerful genetic model system to study temperature control of the clock. In this proposed research, we will use genetic and genomic approaches as well as real-time imaging in C elegans to investigate the molecular mechanisms and neural pathways underlying temperature control ofthe circadian clock.
Specific Aim 1 will develop a new real-time imaging system for recording and quantifying circadian rhythmicity in behavior and gene expression in freely moving C elegans. This imaging will be useful for mutant analysis and genetic screening of clock mutants.
Specific Aim 2 will define the sensory neuron types that transmit temperature information to the clock(s). This will aid in understanding the sensory pathways that process and integrate environmental information to the clock.
Specific Aim 3 will define the molecular components ofthe clock. These components are expected to encode for core-clock genes and components that process temperature information from the core-clock to clock-output genes.
Specific Aim 4 will identify circadian genes expressed in single sensory neuron types with mRNAsequencing. Identification ofthe complete set of sensory genes regulated by the C elegans clock will determine the behavioral consequences of differential expression of genes that process environmental information to the clock. Understanding the inner workings ofthe circadian clock in great depth and the impacts on circadian time keeping should provide us with new avenues of treatment or prevention of neurobehavioral consequences of disrupted circadian timing.

Public Health Relevance

Studying the biological clock and impacts on biological time keeping will ultimately lead to strategies for treatment and prevention of neurological disorders where disturbances in timing are important causes of morbidity.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Exploratory Grants (P20)
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Special Emphasis Panel (ZRR1-RI-4)
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University of Nevada Reno
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Leonard, Anne S; Masek, Pavel (2014) Multisensory integration of colors and scents: insights from bees and flowers. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 200:463-74
Kim, Minkyung; Farmer, W Todd; Bjorke, Brielle et al. (2014) Pioneer midbrain longitudinal axons navigate using a balance of Netrin attraction and Slit repulsion. Neural Dev 9:17
Gruner, Matthew; Nelson, Dru; Winbush, Ari et al. (2014) Feeding state, insulin and NPR-1 modulate chemoreceptor gene expression via integration of sensory and circuit inputs. PLoS Genet 10:e1004707
McCarthy, J Daniel; Caplovitz, Gideon Paul (2014) Color synesthesia improves color but impairs motion perception. Trends Cogn Sci 18:224-6
Masek, Pavel; Reynolds, Lauren A; Bollinger, Wesley L et al. (2014) Altered regulation of sleep and feeding contributes to starvation resistance in Drosophila melanogaster. J Exp Biol 217:3122-32
Peterson, Dwight J; Gurariy, Gennadiy; Dimotsantos, Gabriella G et al. (2014) The steady-state visual evoked potential reveals neural correlates of the items encoded into visual working memory. Neuropsychologia 63:145-53
Wu, Jingwen; Bao, Jianqiang; Kim, Minkyung et al. (2014) Two miRNA clusters, miR-34b/c and miR-449, are essential for normal brain development, motile ciliogenesis, and spermatogenesis. Proc Natl Acad Sci U S A 111:E2851-7
Gözenman, Filiz; Tanoue, Ryan T; Metoyer, Terina et al. (2014) Invalid retro-cues can eliminate the retro-cue benefit: Evidence for a hybridized account. J Exp Psychol Hum Percept Perform 40:1748-54
Janczyk, Markus; Berryhill, Marian E (2014) Orienting attention in visual working memory requires central capacity: decreased retro-cue effects under dual-task conditions. Atten Percept Psychophys 76:715-24
Jones, Kevin T; Gözenman, Filiz; Berryhill, Marian E (2014) Enhanced long-term memory encoding after parietal neurostimulation. Exp Brain Res 232:4043-54

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