The COBRE Core C: Cell Analysis and Sorting is located at the University of Rhode Island, Providence. The role of Core C will be to provide expertise for the design, trouble shooting and analysis of assays for the COBRE investigators. This expertise will be provided by the CMI core director, Loren Fast, Ph.D., who has over 30 years of experience as a cellular immunologist studying immune responses of both human and murine lymphocytes. The CMI core manager, Joe Derosiers, B.S., has over 3 years of experience maintaining and running the core instrumentation.
The second aim i s provide and maintain instrumentation that can be used to measure the responses of immune assays. The instrumentation available for use by the COBRE investigators includes an BD LSRll flow cytometer with four lasers capable of reading at least 12 colors, an AUTOMacs Pro for cell separation, ELISPOT and ELISA readers for obtaining results from these assays, an AKTA Avant FPLC and a controlled rate freezer for storage of cells. We are also proposing to acquire a Miltenyi MACSQuant Analyzer which will be good for routine analysis of sample containing up to 7 colors. This will free up the LSRll for analysis of more complicated samples. The cell-mediated immunity (CMI) core for the Ul 9 TRIAD grant is already in place at University of Rhode Island and the efforts of Core C with synergize with the efforts ofthe CMI core.
The COBRE Core C: Cell Analysis and Sorting will serve as a resource for the other COBRE investigators by providing expert advice as they develop and carry out their experiments and provide special instruments for their use in measuring the results of their experiments.
|McAllaster, Michael R; Sinclair-Davis, Amy N; Hilton, Nicholas A et al. (2016) A unified approach towards Trypanosoma brucei functional genomics using Gibson assembly. Mol Biochem Parasitol 210:13-21|
|Li, Ming; Tucker, Lynne D; Asara, John M et al. (2016) Stem-loop binding protein is a multifaceted cellular regulator of HIV-1 replication. J Clin Invest 126:3117-29|
|Li, Ming; Ramratnam, Bharat (2016) Proteomic Characterization of Exosomes from HIV-1-Infected Cells. Methods Mol Biol 1354:311-26|
|McAllaster, Michael R; Ikeda, Kyojiro N; Lozano-NÃºÃ±ez, Ana et al. (2015) Proteomic identification of novel cytoskeletal proteins associated with TbPLK, an essential regulator of cell morphogenesis in Trypanosoma brucei. Mol Biol Cell 26:3013-29|
|Arsenault, Amanda B; Bliss, Joseph M (2015) Neonatal Candidiasis: New Insights into an Old Problem at a Unique Host-Pathogen Interface. Curr Fungal Infect Rep 9:246-252|
|Li, Ming (2015) Proteomics in the investigation of HIV-1 interactions with host proteins. Proteomics Clin Appl 9:221-34|
|Medin, Carey L; Valois, Sierra; Patkar, Chinmay G et al. (2015) A plasmid-based reporter system for live cell imaging of dengue virus infected cells. J Virol Methods 211:55-62|
|Nixon, Christian P; Cheves, Tracey A; Sweeney, Joseph D (2015) Sulfamethoxazole-induced thrombocytopenia masquerading as posttransfusion purpura: a case report. Transfusion 55:2738-41|