The principal aim of Core D - Luminex High-Throughput Analysis Core is to provide barcode-driven, robot-pipetted high throughput multiplex assays for all four ofthe proposed COBRE projects. Secondary aims include: 1) to provide a standardized tracking database and storage for samples from all scientific projects and 2) to implement a QC plan with documentation for assays performed for all scientific projects. Access to high throughput assay technology is essential for the timely completion of the large quantity of assays required to test the hypotheses contained in the scientific projects. Moreover, by centralizing assay services, we access 1) economies of scale for equipment, supplies and technologist expertise, 2) rigorous application of GLP standards during assay development, and 3) the incorporation of CAP (College of American Pathologists) quality controls (both methods and reagents) for all clinical analytes (w/ commercial QC reagents) and non-clinical analytes (w/ """"""""in house"""""""" QC reagents) on ALL production assay runs. CIHR already has a well-established high-throughput immunoassay core and is a recognized leader in this technology. We have developed multiplexed bead-based assays to measure: inflammatory, nutritional and iron status markers (15-plex and 12-plex assays), antigen and isotype specific antibodies (56-plex for malaria-specific antibodies), and fibrosis (38-plex). For Projects 1 and 2, the Core will develop and rapidly measure inflammatory markers in serum and culture supernatants. For Project 3, the Core will develop and rapidly measure malarial antigen specific, isotype specific antibodies. For Projects 1, 2, and 3, the Core will implement a Quality Control strategy for all assays, including multiplex assays.
Core D will provide bar-code driven, robot pipetted high throughput multiplex assays for all ofthe proposed COBRE projects. Secondary aims include: 1) to provide a standardized tracking database and storage for samples from all scientific projects and 2) to implement a QC plan with documentation for assays performed for all scientific projects.
|Lohman-Payne, Barbara; Gabriel, Benjamin; Park, Sangshin et al. (2018) HIV-exposed uninfected infants: elevated cord blood Interleukin 8 (IL-8) is significantly associated with maternal HIV infection and systemic IL-8 in a Kenyan cohort. Clin Transl Med 7:26|
|Adam, Awadalkareem; Woda, Marcia; Kounlavouth, Sonia et al. (2018) Multiplexed FluoroSpot for the Analysis of Dengue Virus- and Zika Virus-Specific and Cross-Reactive Memory B Cells. J Immunol 201:3804-3814|
|Nixon, Christian P; Satyagraha, Ari W; Baird, Grayson L et al. (2018) Accurate light microscopic diagnosis of South-East Asian ovalocytosis. Int J Lab Hematol 40:655-662|
|Cheruiyot, Collins; Pataki, Zemplen; Williams, Robert et al. (2017) SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells. J Vis Exp :|
|Nixon, Christian P; Prsic, Elizabeth H; Guertin, Christine A et al. (2017) Acquired Factor XIII inhibitor associated with mantle cell lymphoma. Transfusion 57:694-699|
|Nixon, Christina E; Park, Sangshin; Pond-Tor, Sunthorn et al. (2017) Identification of Protective B-Cell Epitopes within the Novel Malaria Vaccine Candidate Plasmodium falciparum Schizont Egress Antigen 1. Clin Vaccine Immunol 24:|
|Barbier, Vincent; Lang, Diane; Valois, Sierra et al. (2017) Dengue virus induces mitochondrial elongation through impairment of Drp1-triggered mitochondrial fission. Virology 500:149-160|
|Smith, Andrew M; Papaleo, Cassandra; Reid, Christopher W et al. (2017) RNA-Seq reveals a central role for lectin, C1q and von Willebrand factor A domains in the defensive glue of a terrestrial slug. Biofouling 33:741-754|
|Li, Ming; Tucker, Lynne D; Asara, John M et al. (2016) Stem-loop binding protein is a multifaceted cellular regulator of HIV-1 replication. J Clin Invest 126:3117-29|
|Nixon, Christian P (2016) Plasmodium falciparum gametocyte transit through the cutaneous microvasculature: A new target for malaria transmission blocking vaccines? Hum Vaccin Immunother 12:3189-3195|
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