This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Seasonal and pandemic influenza A is a leading cause of infectious lung disease in the U.S. and worldwide. The most severe infections of influenza virus involve cells of the lower lung causing a viral pneumonia. One of the lung cell types that can be infected by influenza virus, alveolar macrophages, also play a role in the innate immune response to the infection. Cell proteins from infected alveolar macrophage will be systematically screened using a mass spectrometry based proteomics approach. Stable isotope labeling by amino acids in cell culture (SILAC) will be used to identify proteins that are differentially expressed in influenza virus infected cells compared to uninfected cells. Candidate differentially-expressed proteins from the mass spectrometry results will be validated by western blot analysis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016460-10
Application #
8359819
Study Section
Special Emphasis Panel (ZRR1-RI-7 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
10
Fiscal Year
2011
Total Cost
$19,348
Indirect Cost
Name
University of Arkansas for Medical Sciences
Department
Physiology
Type
Schools of Medicine
DUNS #
122452563
City
Little Rock
State
AR
Country
United States
Zip Code
72205
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