This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this proposed research is to explore similarities and differences in the translational regulation of a subunit of RNA polymerase and a ribosomal protein operon across diverse prokaryotes. The rpoS gene, encoding the alternative sigma factor sigma s, which influences bacterial virulence, is translationally regulated as a response to environmental changes in both Escherichia coli and Borrelia burgdorferi, the causative agent of Lyme disease. The roles of B. burgdorferi rpoS mRNA sequence, length and secondary structure, and the roles of trans-acting regulators, in particular a small non-coding RNA molecule, on translational initiation events will be characterized. In E. coli, the four ribosomal proteins of the alpha operon are translationally repressed by ribosomal protein S4, one of the proteins encoded in the operon. In Prochlorococcus marinus, Synechococcus sp. WH8102 and B. burgdorferi, rpsD, encoding S4, maps outside of the operon, suggesting a different mechanism of regulation. Both in vitro assays, using cell free extracts, and in vivo expression assays will be used to identify the cis-acting and trans-acting factors important in the translational regulation of the ? operon in these diverse bacteria and to elucidate their mechanism of translational regulation.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016463-11
Application #
8360309
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
11
Fiscal Year
2011
Total Cost
$140,567
Indirect Cost
Name
Mount Desert Island Biological Lab
Department
Type
DUNS #
077470003
City
Salsbury Cove
State
ME
Country
United States
Zip Code
04672
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