This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Examination of the ecology complemented with functional genomics of endocrine disruption in a salamander will allow development of an amphibian ED bioassy system that can be used in human environmental health policy. Use of microarray analysis and subsequent in situ hybridization can identify differential gene expression associated with atrazine exposure. When this genomics approach is complemented by ecological manipulations of a broad array of conditions, it allows for identification of a likely set of hypotheses for what conditions are resulting in ED-mediated up- and down-regulation of genes. Atrazine and estrogen have obvious effects on sexual maturation, but also interact with the hormones that regulate amphibian metamorphosis. The work described in this proposal is designed to test the hypothesis that the action of two endocrine disruptors (atrazine and estrogen) found commonly in the environment will interact with the normal ecological cues experienced by larval salamanders. The result of such interactions of EDs and ecology will be larger-than-expected effects of sub-lethal concentration of EDs on mortality, growth and metamorphic and sexual development of the larval salamanders.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016471-11
Application #
8360052
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
11
Fiscal Year
2011
Total Cost
$138,408
Indirect Cost
Name
University of North Dakota
Department
Pathology
Type
Schools of Medicine
DUNS #
102280781
City
Grand Forks
State
ND
Country
United States
Zip Code
58202
Beachy, Christopher K; Ryan, Travis J; Bonett, Ronald M (2017) How Metamorphosis Is Different in Plethodontids: Larval Life History Perspectives on Life-Cycle Evolution. Herpetologica 73:252-258
Keller, Christopher P (2017) Leaf expansion in Phaseolus: transient auxin-induced growth increase. Physiol Plant 130:580-589
Wold, Marissa; Beckmann, Myranda; Poitra, Shelby et al. (2017) The longitudinal effects of early developmental cadmium exposure on conditioned place preference and cardiovascular physiology in zebrafish. Aquat Toxicol 191:73-84
Zhou, Lun; Liu, Jielin; Xiang, Menglan et al. (2017) Gata4 potentiates second heart field proliferation and Hedgehog signaling for cardiac septation. Proc Natl Acad Sci U S A 114:E1422-E1431
Fu, Q; Olson, P; Rasmussen, D et al. (2016) A short-term transition from a high-fat diet to a normal-fat diet before pregnancy exacerbates female mouse offspring obesity. Int J Obes (Lond) 40:564-72
Johnson, Kristin M; Woods, Rebecca J (2016) Give Me a Hand: Adult Involvement During Object Exploration Affects Object Individuation in Infancy. Infant Child Dev 25:406-425
Zhang, Ke K; Xiang, Menglan; Zhou, Lun et al. (2016) Gene network and familial analyses uncover a gene network involving Tbx5/Osr1/Pcsk6 interaction in the second heart field for atrial septation. Hum Mol Genet 25:1140-51
Mersereau, Eric J; Boyle, Cody A; Poitra, Shelby et al. (2016) Longitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafish. Int J Mol Sci 17:
Ihli, Lori S; Beachy, Christopher K (2016) Experimental Analysis of Allocation during Larval Development in Ambystomatid Salamanders. Herpetologica 72:1-5
Rebel, Andrew A; Urquhart, Siri A; Puig, Kendra L et al. (2015) Brain changes associated with thromboxane receptor antagonist SQ 29,548 treatment in a mouse model. J Neurosci Res 93:1279-92

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