This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff.
This research aims to determine the molecular mechanism of action of the A. nidulans transcriptional corepressor NmrA, a key component of an important regulatory system governing nutritional response. NmrA represses during nitrogen sufficiency the activity of the AreA GATA transcription factor. AreA activates expression of nitrogen metabolic genes during nitrogen limitation or nitrogen starvation. NmrA is thought to inhibit AreA activity by direct protein-protein interaction but the mechanism of NmrA action is not understood. This proposal will investigate how the corepressor NmrA regulates AreA activity. The key aims are (1) to establish whether NmrA acts as a regulatory NAD-dependent protein deacetylase enzyme, (2) to identify NmrA interactors by proteomics, and (3) to identify a new gene involved in NmrA-mediated repression. Objective 1: Does NmrA mediate repression by functioning as a regulatory enzyme? 1.1 Detection of NAD-dependent deacetylase activity using the Fluor-de-lys"""""""" substrate. Objective 2: Identification of proteins that interact with the NmrA repressor using a proteomics approach. 2.1 Biacore capture of proteins that interact with NmrA. Objective 3: Characterization of a suppressor of an NmrA overexpression phenotype. 3.1 Determination of the sequence change in a mutant affected in a novel gene affecting NmrA function by massively parallel sequencing. 3.2 Functional confirmation of mutations identified in the unknown gene. 3.3 Initial molecular genetic analysis of the new player in NmrA-mediated repression.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016475-11
Application #
8359746
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
11
Fiscal Year
2011
Total Cost
$60,500
Indirect Cost
Name
University of Kansas
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160
Pang, Xiao-Yan; Wang, Suya; Jurczak, Michael J et al. (2017) Retinol saturase modulates lipid metabolism and the production of reactive oxygen species. Arch Biochem Biophys 633:93-102
Barton, Janice S; Schomacker, Rachel (2017) Comparative protein profiles of the Ambrosia plants. Biochim Biophys Acta 1865:633-639
Dowdell, Alexander S; Murphy, Maxwell D; Azodi, Christina et al. (2017) Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome Reveals a Compartmentalization Bias toward the Bacterial Surface. J Bacteriol 199:
Chakrabarti, Rima S; Ingham, Sally A; Kozlitina, Julia et al. (2017) Variability of cholesterol accessibility in human red blood cells measured using a bacterial cholesterol-binding toxin. Elife 6:
Bowden, John A; Heckert, Alan; Ulmer, Candice Z et al. (2017) Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma. J Lipid Res 58:2275-2288
Guilford, B L; Ryals, J M; Lezi, E et al. (2017) Dorsal Root Ganglia Mitochondrial Biochemical Changes in Non-diabetic and Streptozotocin-Induced Diabetic Mice Fed with a Standard or High-Fat Diet. J Neurol Neurosci 8:
Rogers, Robert S; Tungtur, Sudheer; Tanaka, Tomohiro et al. (2017) Impaired Mitophagy Plays a Role in Denervation of Neuromuscular Junctions in ALS Mice. Front Neurosci 11:473
Moon, Sanghee; Schmidt, Marshall; Smirnova, Irina V et al. (2017) Qigong Exercise May Reduce Serum TNF-? Levels and Improve Sleep in People with Parkinson's Disease: A Pilot Study. Medicines (Basel) 4:
Pook, Victoria G; Nair, Meera; Ryu, KookHui et al. (2017) Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots. Sci Rep 7:5714
Kania-Korwel, Izabela; Wu, Xianai; Wang, Kai et al. (2017) Identification of lipidomic markers of chronic 3,3',4,4',5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver. Toxicology 390:124-134

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