This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff.
The aim of this research is to develop a chemical sensor that is selective and specific for Dopamine. Dopamine is an important neurotransmitter, a chemical messenger between the nerve cells in the mammalian brain. It also plays an important role in the function of the renal, hormonal and cardiovascular systems. Therefore the development of dopamine quantification in blood and other biological systems is very important. The specific binding sites for dopamine will be created in polymers during the imprinting process. Molecular Imprinted polymer (MIP) microspheres will be synthesized via precipitation polymerization. The dopamine print molecule, the principal monomer, N-Isopropyl acrylamide or vinyl caprolactum, and the functional monomer methacrylic acid will be dispersed in the solvent by sonication. The crosslinker, stabilizer and initiator will then be added to this mixture. The functional monomer will form a complex with the dopamine print molecule and following polymerization, their functional groups will be held in position by the crosslinked polymeric structure. Removal of the print molecule will reveal sites that have a molecular memory, which will allow the polymer to selectively rebind the dopamine analyte.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016479-11
Application #
8360645
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
11
Fiscal Year
2011
Total Cost
$118,600
Indirect Cost
Name
University of South Dakota
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
929930808
City
Vermillion
State
SD
Country
United States
Zip Code
57069
Rozenblat, Vanja; Ong, Deborah; Fuller-Tyszkiewicz, Matthew et al. (2017) A systematic review and secondary data analysis of the interactions between the serotonin transporter 5-HTTLPR polymorphism and environmental and psychological factors in eating disorders. J Psychiatr Res 84:62-72
Oleas, Gabriela; Callegari, Eduardo; Sepúlveda, Romina et al. (2017) Heterologous expression, purification and characterization of three novel esterases secreted by the lignocellulolytic fungus Penicillium purpurogenum when grown on sugar beet pulp. Carbohydr Res 443-444:42-48
Zhang, Lei; Hapon, Maria B; Goyeneche, Alicia A et al. (2016) Mifepristone increases mRNA translation rate, triggers the unfolded protein response, increases autophagic flux, and kills ovarian cancer cells in combination with proteasome or lysosome inhibitors. Mol Oncol 10:1099-117
Callegari, Eduardo A (2016) Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two-Dimensional Liquid Chromatography and Tandem Mass Spectrometry. Methods Mol Biol 1366:131-148
Oleas, Gabriela; Callegari, Eduardo; Sepulveda, Romina et al. (2016) Properties of Two Novel Esterases Identified from Culture Supernatant of Penicillium purpurogenum Grown on Sugar Beet Pulp. Insights Enzym Res 1:
Bonilla, José Oscar; Callegari, Eduardo Alberto; Delfini, Claudio Daniel et al. (2016) Simultaneous chromate and sulfate removal by Streptomyces sp. MC1. Changes in intracellular protein profile induced by Cr(VI). J Basic Microbiol :
Herrera, Andrea L; Huber, Victor C; Chaussee, Michael S (2016) The Association between Invasive Group A Streptococcal Diseases and Viral Respiratory Tract Infections. Front Microbiol 7:342
Zhang, Fan; Hartnett, Sigurd; Sample, Alex et al. (2016) High fat diet induced alterations of atrial electrical activities in mice. Am J Cardiovasc Dis 6:1-9
Haag, Nichole; Velk, Kimberly; McCune, Tyler et al. (2015) Bioinformatics and Molecular Biological Characterization of a Hypothetical Protein SAV1226 as a Potential Drug Target for Methicillin/Vancomycin-Staphylococcus aureus Infections. World Acad Sci Eng Technol 9:587-591
Wu, Steven C; O?Connell, Timothy D (2015) A nuclear option? G-protein coupled receptors at the nucleus in cardiac myocytes. J Cardiovasc Pharmacol 65:89-90

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