This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Core Facility for Flow Cytometry and Cell Sorting provides enabling technology not only for OMRF, but for others on the Health Sciences Campus and throughout the state of Oklahoma. Other institutions have analyzers, and two are able to sort cells. However, OMRF's facility is uniquely state of the art and has often helped others with technical issues. Investigators in this COBRE will have priority access not only to the instruments, but also to training. This is important because we have learned that scientists get maximum yield from their experiments when they are at least able to analyze their own data. Some fellows even learn how to operate the FACSAria, but this is done under close supervision by Facility technicians. Training goes even further by encouraging experienced users to share multi-color staining and bead separation technology with new users. The projects of Drs. Lijun Xia, Courtney Griffin, Jana Barlic, Hong Chen and Tim Griffin all require multiparameter flow cytometry and/or cell sorting. It will be necessary to manipulate rare cell types from liver, blood, adipose tissues, skin and bone marrow. These include lymphatic endothelial cells, blood endothelial cells, monocytes, macrophages and other leukocytes. Many OMRF scientists are already experienced with enumeration and sorting of extremely rare populations such as stem cells. Additionally, they use the facility to determine the extent of chimerism in transplanted mice and the efficacy of expression or knockdown constructs. Thus, everything that has been proposed or is likely to be done is within the capabilities of the Core Facility. As part of the mentoring function, new and more informative experimental protocols will be suggested to COBRE investigators. Finally, the Core Facility will help to recruit additional talented scientists by providing them with essential, state-of-the-art support.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR018758-07
Application #
8364982
Study Section
Special Emphasis Panel (ZRR1-CR-B (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
7
Fiscal Year
2011
Total Cost
$120,288
Indirect Cost
Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
077333797
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104
Dong, Yunzhou; Fernandes, Conrad; Liu, Yanjun et al. (2017) Role of endoplasmic reticulum stress signalling in diabetic endothelial dysfunction and atherosclerosis. Diab Vasc Dis Res 14:14-23
Bergstrom, K; Fu, J; Johansson, M E V et al. (2017) Core 1- and 3-derived O-glycans collectively maintain the colonic mucus barrier and protect against spontaneous colitis in mice. Mucosal Immunol 10:91-103
Barboza, Erika; Hudson, Joanna; Chang, Wan-Pin et al. (2017) Profibrotic Infrapatellar Fat Pad Remodeling Without M1 Macrophage Polarization Precedes Knee Osteoarthritis in Mice With Diet-Induced Obesity. Arthritis Rheumatol 69:1221-1232
Bergstrom, Kirk; Liu, Xiaowei; Zhao, Yiming et al. (2016) Defective Intestinal Mucin-Type O-Glycosylation Causes Spontaneous Colitis-Associated Cancer in Mice. Gastroenterology 151:152-164.e11
Rahman, H N Ashiqur; Wu, Hao; Dong, Yunzhou et al. (2016) Selective Targeting of a Novel Epsin-VEGFR2 Interaction Promotes VEGF-Mediated Angiogenesis. Circ Res 118:957-969
Fu, Yao; Kinter, Michael; Hudson, Joanna et al. (2016) Aging Promotes Sirtuin 3-Dependent Cartilage Superoxide Dismutase 2 Acetylation and Osteoarthritis. Arthritis Rheumatol 68:1887-98
Griffin, Timothy M; Humphries, Kenneth M; Kinter, Michael et al. (2016) Nutrient sensing and utilization: Getting to the heart of metabolic flexibility. Biochimie 124:74-83
Fu, Yao; Huebner, Janet L; Kraus, Virginia B et al. (2016) Effect of Aging on Adipose Tissue Inflammation in the Knee Joints of F344BN Rats. J Gerontol A Biol Sci Med Sci 71:1131-40
Herlea-Pana, Oana; Yao, Longbiao; Heuser-Baker, Janet et al. (2015) Chemokine receptors CXCR2 and CX3CR1 differentially regulate functional responses of bone-marrow endothelial progenitors during atherosclerotic plaque regression. Cardiovasc Res 106:324-37
Dong, Yunzhou; Wu, Hao; Rahman, H N Ashiqur et al. (2015) Motif mimetic of epsin perturbs tumor growth and metastasis. J Clin Invest 125:4349-64

Showing the most recent 10 out of 63 publications