This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent clinical success in bladder tissue engineering demonstrated the feasibility of this technology to combine synthetic polymer scaffolds with host cells for bladder reconstruction. Yet, there remain several unresolved issues. For example, in-vitro culturing of bladder smooth muscle cells for a prolonged time period results in de-differentiation and loss of the contractile phenotype of these cells. Our group previously demonstrated that 3D cultures of bladder SMCs subjected to sustained tension exhibited significantly greater levels of contractile phenotype markers compared to the no-tension control. We hypothesize that exposure of 3D culture of bladder SMCs to appropriate mechanical stimuli leads to guided cell growth and retention of contractile phenotype. The long-term objective of the present study is to develop both biomaterials and a bioreactor system that guide SMC growth for use in the tissue engineering applications.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR021949-03
Application #
8360199
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
3
Fiscal Year
2011
Total Cost
$111,751
Indirect Cost
Name
Clemson University
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
042629816
City
Clemson
State
SC
Country
United States
Zip Code
29634
Liu, Honghai; Qin, Wan; Wang, Zhonghai et al. (2016) Disassembly of myofibrils and potential imbalanced forces on Z-discs in cultured adult cardiomyocytes. Cytoskeleton (Hoboken) 73:246-57
Huang, Ting; Wang, Zhonghai; Wei, Lina et al. (2016) Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial. J Mater Sci Technol 32:89-96
Kuang, Serena Y; Yang, Xiaoqi; Wang, Zhonghai et al. (2016) How Microelectrode Array-Based Chick Forebrain Neuron Biosensors Respond to Glutamate NMDA Receptor Antagonist AP5 and GABAA Receptor Antagonist Musimol. Sens Biosensing Res 10:9-14
Olsen, T R; Mattix, B; Casco, M et al. (2015) Manipulation of cellular spheroid composition and the effects on vascular tissue fusion. Acta Biomater 13:188-98
Nosoudi, Nasim; Nahar-Gohad, Pranjal; Sinha, Aditi et al. (2015) Prevention of abdominal aortic aneurysm progression by targeted inhibition of matrix metalloproteinase activity with batimastat-loaded nanoparticles. Circ Res 117:e80-9
Thyparambil, Aby A; Wei, Yang; Latour, Robert A (2015) Experimental characterization of adsorbed protein orientation, conformation, and bioactivity. Biointerphases 10:019002
Kuang, Serena Y; Huang, Ting; Wang, Zhonghai et al. (2015) Establishment of a Long-Term Chick Forebrain Neuronal Culture on a Microelectrode Array Platform. RSC Adv 5:56244-56254
Yang, Huaxiao; Borg, Thomas K; Liu, Honghai et al. (2015) Interactive relationship between basement-membrane development and sarcomerogenesis in single cardiomyocytes. Exp Cell Res 330:222-32
Bae, Sooneon; Lee, Ho-Joon; Lee, Jeoung Soo et al. (2015) Cell-Mediated Dexamethasone Release from Semi-IPNs Stimulates Osteogenic Differentiation of Encapsulated Mesenchymal Stem Cells. Biomacromolecules 16:2757-65
Thyparambil, Aby A; Wei, Yang; Latour, Robert A (2015) Evaluation of the Effectiveness of Surfactants and Denaturants to Elute and Denature Adsorbed Protein on Different Surface Chemistries. Langmuir 31:11814-24

Showing the most recent 10 out of 104 publications