The major objective of the University of Alabama (DAB) Center for AIDS Research (CFAR) Flow Cytometry Core is to provide state-of-the-art flow cytometry services to support AIDS research programs. Flow cytometry is a unique and powerful technology for analyzing the fluorescent properties of particles and can be readily applied to determining the phenotype and function of cells, as well as utilized for isolating defined cell populations by sorting. In recent years the use of flow cytometric analyses in HIV research has become ever more prominent as newer approaches for dissecting the interactions between HIV and cells of the immune system have been developed;fluorescent based reporter systems have been harnessed to monitor virus replication, reactivation, and recombination;and sophisticated technological advances in instrumentation have provided new platforms for high-speed polychromatic analyses and cell sorting. The CFAR flow cytometry core is, therefore, a vital and versatile resource for promoting and facilitating AIDS research. The flow cytometry core's mission is to maximize the benefits of this technology by providing the necessary instrumentation within a well organized, centralized, facility that is capable of handling potentially infectious material. It provides training opportunities and consultations, and it also serves as a conduit for fostering interactions between investigators and laboratory personnel with diverse interests and expertise. During the previous funding period the CFAR flow cytometry core has undergone a significant equipment upgrade, with the installation of a BDFACSAria cell sorter as well as a BD-LSR-II analytical flow cytometer. The flow cytometry core has also improved its visibility and accessibility as well as enhanced education and training initiatives. As a result of these endeavors the usage of the flow cytometry core has increased more than three-fold since 2002. The flow cytometry core has supported the research activities of 184 different individuals from 69 Principle Investigators laboratories and has contributed to basic, clinical, and translational initiatives in the areas of viral pathogenesis, anti-viral research, cellular immunity, and vaccine design. The core has supported the activities of 88 NIH grants and contributed to 172 publications The aims of the flow cytometry core are to now build upon our progress-to-date by further enhancing services, and education and training activities so that users are empowered to take full advantage of the sophisticated instrumentation that is available. These efforts include, based on a needs assessment analysis, the installation of a third flow cytometer and a BioPlex flow cytometer for multiplexed analysis of biomolecules (e.g. cytokines) in the beginning of 2008. We also aim to promote innovation by working with users, and fostering collaborations and crosscore partnerships that encourage cutting-edge AIDS related research activities.

Public Health Relevance

Flow cytometric analysis has emerged as a crucial tool to study how HIV-1 infection causes disease and death. It has and will continue to be instrumental to develop a deeper understanding towards better treatment strategies and potentially a vaccine against HIV-1. The CFAR Flow Cytometry Core thus provides an essential bridge between basic research efforts and translational clinical investigation.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Center Core Grants (P30)
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Special Emphasis Panel (ZAI1-SV-A)
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University of Alabama Birmingham
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Salazar-Gonzalez, Jesus F; Salazar, Maria G; Tully, Damien C et al. (2016) Use of Dried Blood Spots to Elucidate Full-Length Transmitted/Founder HIV-1 Genomes. Pathog Immun 1:129-153
Abedini-Nassab, Roozbeh; Joh, Daniel Y; Van Heest, Melissa et al. (2016) Magnetophoretic Conductors and Diodes in a 3D Magnetic Field. Adv Funct Mater 26:4026-4034
Dalecki, Alex G; Malalasekera, Aruni P; Schaaf, Kaitlyn et al. (2016) Combinatorial phenotypic screen uncovers unrecognized family of extended thiourea inhibitors with copper-dependent anti-staphylococcal activity. Metallomics 8:412-21
Pettit, April C; Mendes, Adell; Jenkins, Cathy et al. (2016) Timing of Antiretroviral Treatment, Immunovirologic Status, and TB Risk: Implications for Testing and Treatment. J Acquir Immune Defic Syndr 72:572-8
Friedman, Gregory K; Moore, Blake P; Nan, Li et al. (2016) Pediatric medulloblastoma xenografts including molecular subgroup 3 and CD133+ and CD15+ cells are sensitive to killing by oncolytic herpes simplex viruses. Neuro Oncol 18:227-35
Demark-Wahnefried, Wendy; Nix, Jeffery W; Hunter, Gary R et al. (2016) Feasibility outcomes of a presurgical randomized controlled trial exploring the impact of caloric restriction and increased physical activity versus a wait-list control on tumor characteristics and circulating biomarkers in men electing prostatectomy for BMC Cancer 16:61
Merlin, Jessica S; Tamhane, Ashutosh; Starrels, Joanna L et al. (2016) Factors Associated with Prescription of Opioids and Co-prescription of Sedating Medications in Individuals with HIV. AIDS Behav 20:687-98
Khan, Shahzada; Woodruff, Erik M; Trapecar, Martin et al. (2016) Dampened antiviral immunity to intravaginal exposure to RNA viral pathogens allows enhanced viral replication. J Exp Med 213:2913-2929
Kumar, Ashish; Zhang, Jennifer; Tallaksen-Greene, Sara et al. (2016) Allelic series of Huntington's disease knock-in mice reveals expression discorrelates. Hum Mol Genet 25:1619-36
Qin, Hongwei; Buckley, Jessica A; Li, Xinru et al. (2016) Inhibition of the JAK/STAT Pathway Protects Against α-Synuclein-Induced Neuroinflammation and Dopaminergic Neurodegeneration. J Neurosci 36:5144-59

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