The DNA Sequencing and Analysis Core has been in operation for the past twenty-one years and has provided CFAR members with state-of-the-art automated sequencing (DNA Sequencing Facility) and computer analysis capabilities (Molecular and Genetic Bioinformatics Facility). Core functions have included development of new methodologies for the automated sequencing process, training of investigators in the use of sequence analysis tools, development of new molecular biology approaches to characterize HIV/SIV genetic diversity, as well as supporting multidisciplinary and multi-institutional projects and collaborations. In the last budget period, the DNA Sequencing Facility has supported 62 CFAR investigators and provided essential services for over 100 AIDS related grants and contracts. 194 million base pairs of primary sequence were determined, generating over $2,100,000 in user charge-backs. Recently implemented automation has increased core efficiency and allowed to reduce user chargebacks from $8 to $6 per sequencing reaction. During the same time period, the Molecular and Genetic Bioinformatics Facility has actively supported over 30 CFAR investigators, and provided essential services for 62 AIDS related grants and contracts.
Specific Aims of the Core are: 1. To continue to provide automated DNA sequencing capabilities to CFAR members through the availability and maintenance of dedicated Applied Biosystems DNA Sequencers with capillary electrophoresis systems. 2. To establish new methods, technologies and reagents to assist investigators in the characterization of HIV/SIV genetic diversity. 3. To provide and maintain a comprehensive set of modem bioinformatic databases and analytical tools for the analysis of genetic information, including gene and genomic sequences. 4. To provide technical support and training in the use of these bioinformatic resources.
Studies of HIV pathogenesis, gene functions, immune responses and escape, vaccine development and drug discovery all necessitate access to rapid nucleotide sequence determinations and sophisticated bioinformatics analyses. The DNA Sequencing and Analysis Core thus supports the entire spectrum of AIDS related basic science research programs and has established itself as an integral component of the AIDS Center. Support of the DNA Sequencing Core is essential for the continuing success of AIDS investigators at UAB.
|Rice, Whitney S; Logie, Carmen H; Napoles, Tessa M et al. (2018) Perceptions of intersectional stigma among diverse women living with HIV in the United States. Soc Sci Med 208:9-17|
|Elion, Richard A; Althoff, Keri N; Zhang, Jinbing et al. (2018) Recent Abacavir Use Increases Risk of Type 1 and Type 2 Myocardial Infarctions Among Adults With HIV. J Acquir Immune Defic Syndr 78:62-72|
|Fredericksen, R J; Gibbons, L; Brown, S et al. (2018) Medication understanding among patients living with multiple chronic conditions: Implications for patient-reported measures of adherence. Res Social Adm Pharm 14:540-544|
|Stoll, Matthew L; Weiss, Pamela F; Weiss, Jennifer E et al. (2018) Age and fecal microbial strain-specific differences in patients with spondyloarthritis. Arthritis Res Ther 20:14|
|Subramaniam, Akila; Van Der Pol, William J; Ptacek, Travis et al. (2018) Midtrimester microbial DNA variations in maternal serum of women who experience spontaneous preterm birth. J Matern Fetal Neonatal Med :1-9|
|Kay, Emma S; Rice, Whitney S; Crockett, Kaylee B et al. (2018) Experienced HIV-Related Stigma in Health Care and Community Settings: Mediated Associations With Psychosocial and Health Outcomes. J Acquir Immune Defic Syndr 77:257-263|
|Xu, Wanli; Luo, Zhenwu; Alekseyenko, Alexander V et al. (2018) Distinct systemic microbiome and microbial translocation are associated with plasma level of anti-CD4 autoantibody in HIV infection. Sci Rep 8:12863|
|Smith, Samuel R; Schaaf, Kaitlyn; Rajabalee, Nusrah et al. (2018) The phosphatase PPM1A controls monocyte-to-macrophage differentiation. Sci Rep 8:902|
|Wang, Yong; Schafer, Cara C; Hough, Kenneth P et al. (2018) Myeloid-Derived Suppressor Cells Impair B Cell Responses in Lung Cancer through IL-7 and STAT5. J Immunol 201:278-295|
|Jones, Robert B; Dorsett, Kaitlyn A; Hjelmeland, Anita B et al. (2018) The ST6Gal-I sialyltransferase protects tumor cells against hypoxia by enhancing HIF-1? signaling. J Biol Chem 293:5659-5667|
Showing the most recent 10 out of 955 publications