Under the direction of Dr. John D. Atlman, the mission of Core H, the Emory CFAR Immunology Core Laboratory (ICL) is to provide the Emory community with the highest quality assessments of immunological function necessary for the study of the pathogenesis, treatment, and prevention of immunodeficiency virus infections in humans and non-human primates. The ICL will achieve this mission through the following specific aims: 1. Flow cytometry. We will maintain a state-of-the-art flow cytometry facility, providing users with access to well maintained and calibrated instruments;"full service" flow cytometry services;cell sorting, including at the BSL-3 level;and training in instrument operation, panel design and data analysis. 2. Antibody responses. We will provide a carefully selected set of services that do not duplicate activities that are standard in potential client laboratories. We will focus on binding antibodies using ELISA and ASC assays (antibody secreting cells) and on isolation of human and non-human primate monoclonal antibodies derived from mRNA isolated for sorted single cells. 3. Luminex analysis. We will provide multiplexed assays of cytokines in human and rhesus samples. 4. PBMC repository. We will continue our rhesus PBMC repository, and, in collaboration with Core D, add a new repository of highly characterized human PBMC samples derived from leukapharesis procedures. 5. T cell responses. We will continue to provide ELISpot and ICS assays, and we will add innovative new approaches to antigen delivery for T cell assays using viral vectors. We will help users relieve bottlenecks in MHC tetramer analysis by providing novel high-throughput methods for tetramer construction and by providing users with combinatorial tetramers including fluorophores that are not available from other sources. 6. We will continue to promote immunological assay education and training opportunities for CFAR investigators, the AIDS research community in Atlanta, and other National and International AIDS researchers.

Public Health Relevance

Through the provision of advanced immunological assays, the numerous projects supported by Core H will be instrumental in developing novel HIV vaccines-both preventative and therapeutic-and in developing a better understanding of AIDS pathogenesis.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30AI050409-16
Application #
8704211
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
16
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Emory University
Department
Type
DUNS #
City
Atlanta
State
GA
Country
United States
Zip Code
30322
Cooper, Hannah L F; Linton, Sabriya; Haley, Danielle F et al. (2015) Changes in Exposure to Neighborhood Characteristics are Associated with Sexual Network Characteristics in a Cohort of Adults Relocating from Public Housing. AIDS Behav 19:1016-30
Jennings, Larissa; Rompalo, Anne M; Wang, Jing et al. (2015) Prevalence and correlates of knowledge of male partner HIV testing and serostatus among African-American women living in high poverty, high HIV prevalence communities (HPTN 064). AIDS Behav 19:291-301
Hulland, Erin N; Brown, Jennifer L; Swartzendruber, Andrea L et al. (2015) The association between stress, coping, and sexual risk behaviors over 24 months among African-American female adolescents. Psychol Health Med 20:443-56
Stephenson, Rob; White, Darcy; Darbes, Lynae et al. (2015) HIV testing behaviors and perceptions of risk of HIV infection among MSM with main partners. AIDS Behav 19:553-60
Siegler, Aaron J; Sullivan, Patrick S; de Voux, Alex et al. (2015) Exploring repeat HIV testing among men who have sex with men in Cape Town and Port Elizabeth, South Africa. AIDS Care 27:229-34
Cho, Jong Hyun; Bondana, Lavanya; Detorio, Mervi A et al. (2014) Synthesis and antiviral evaluation of 2-amino-6-carbamoylpurine dioxolane nucleoside derivatives and their phosphoramidates prodrugs. Bioorg Med Chem 22:6665-71
Lippman, Sheri A; Veloso, Valdiléa G; Buchbinder, Susan et al. (2014) Over-the-counter human immunodeficiency virus self-test kits: time to explore their use for men who have sex with men in Brazil. Braz J Infect Dis 18:239-44
Mustanski, Brian; Ryan, Daniel T; Sanchez, Travis et al. (2014) Effects of messaging about multiple biomedical and behavioral HIV prevention methods on intentions to use among US MSM: results of an experimental messaging study. AIDS Behav 18:1651-60
Wall, Kristin M; Sullivan, Patrick S; Kleinbaum, David et al. (2014) Actor-partner effects associated with experiencing intimate partner violence or coercion among male couples enrolled in an HIV prevention trial. BMC Public Health 14:209
Smith, Kristen L Jurcic; Saini, Divey; Bardarov, Svetoslav et al. (2014) Reduced virulence of an extensively drug-resistant outbreak strain of Mycobacterium tuberculosis in a murine model. PLoS One 9:e94953

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