The Einstein/MMC CFAR Virology Core provides essential and highly used services to CFAR investigators that supply the infrastructure, resources, assays and techniques that permit Einstein/MMC CFAR basic and clinical investigators to investigate different aspects of HIV-1 replication in vitro and in vivo. This greatly facilitates their research efforts by allowing them to quickly and efficiently perform experiments that they could not otherwise do. For investigators who have experience in handling HIV-1, the BSLS/Virology core provides a dedicated BSL3 facility that contains the essential equipment for culturing HIV-1 under the appropriate biosafety conditions. For investigators whose research program requires them to culture HIV-1 but who do not have experience culturing HIV-1, the BSL3/Virology Core trains them in the various techniques and protocols required to safely handle and reliably culture HIV-1. Regular workshops are presented to teach investigators techniques for the measurement of p24 antigen by ELISA, co-culture of HIV-1 from PBMCs, performance of HIV-1 fusion assays using luciferase reporter pseudotyped HIV-1, measurement of plasma HIV-1 RNA levels, and determining viral titers using reporter cell lines. For clinical investigators, the core provides up-to-date, clinically relevant virological assays such as the quantification of plasma HIV-1 RNA and HCV RNA, isolation and phenotypic analysis of HIV, and isolation and storage of PBMC, plasma and/or serum from study patients. The Core also provides support for a wide range of molecular biology techniques including custom vector and plasmid design and construction, DNA sub-cloning and amplification, site-directed mutagenesis, large-scale expression of recombinant HIV, human or other pathogen proteins, siRNA interference construct design, and the heteroduplex mobility assay.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
3P30AI051519-10S1
Application #
8628351
Study Section
Special Emphasis Panel (ZAI1-EC-A)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
10
Fiscal Year
2013
Total Cost
$114,339
Indirect Cost
$40,585
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Rao, Vasudev R; Neogi, Ujjwal; Eugenin, Eliseo et al. (2014) The gp120 protein is a second determinant of decreased neurovirulence of Indian HIV-1C isolates compared to southern African HIV-1C isolates. PLoS One 9:e107074
Singh, M; Quispe-Tintaya, W; Chandra, D et al. (2014) Direct incorporation of the NKT-cell activator ?-galactosylceramide into a recombinant Listeria monocytogenes improves breast cancer vaccine efficacy. Br J Cancer 111:1945-54
Jelen, Mateja M; Chen, Zigui; Kocjan, Boštjan J et al. (2014) Global genomic diversity of human papillomavirus 6 based on 724 isolates and 190 complete genome sequences. J Virol 88:7307-16
Tarassishin, Leonid; Suh, Hyeon-Sook; Lee, Sunhee C (2014) LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14. Glia 62:999-1013
Strickler, Howard D; Martinson, Jeffrey; Desai, Seema et al. (2014) The relation of plasmacytoid dendritic cells (pDCs) and regulatory T-cells (Tregs) with HPV persistence in HIV-infected and HIV-uninfected women. Viral Immunol 27:20-5
Tarassishin, Leonid; Casper, Diana; Lee, Sunhee C (2014) Aberrant expression of interleukin-1? and inflammasome activation in human malignant gliomas. PLoS One 9:e103432
Prados-Rosales, Rafael; Carreño, Leandro J; Batista-Gonzalez, Ana et al. (2014) Mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of Mycobacterium tuberculosis. MBio 5:e01921-14
Stec, Jozef; Vilchèze, Catherine; Lun, Shichun et al. (2014) Biological evaluation of potent triclosan-derived inhibitors of the enoyl-acyl carrier protein reductase InhA in drug-sensitive and drug-resistant strains of Mycobacterium tuberculosis. ChemMedChem 9:2528-37
Benning, Lorie; Golub, Elizabeth T; Anastos, Kathryn et al. (2014) Comparison of lower genital tract microbiota in HIV-infected and uninfected women from Rwanda and the US. PLoS One 9:e96844
Shuter, Jonathan; Moadel, Alyson B; Kim, Ryung S et al. (2014) Self-efficacy to quit in HIV-infected smokers. Nicotine Tob Res 16:1527-31

Showing the most recent 10 out of 381 publications