The Comprehensive Flow Cytometry Core (CFCC) is directed by Dr. John D. Mountz with assistance of Co-Directors, Drs.Troy Randall and Olaf Kutsch. The goal of the CFCC is to enhance the productivity of the research base of the UAB Rheumatic Disease Core Center (RDCC) through state-of-the-art flow cytometry and cell separation technologies. To accomplish this, the Core provides the equipment, service and expertise necessary for the application of flow cytometry and related technologies to cell analyses and cell purification at a reasonable cost. These services play a key role in studies of disease pathogenesis and identification of potential therapeutic targets, as well as in analysis of determinants of disease susceptibility and drug responsiveness, and pre-clinical testing of potential therapeutic reagents.
Our Specific Aims are: 1 Service. To improve service by continued improvements of our equipment, through enhanced sophistication of our user base, optimal efficiency of sample analysis, rigorous quality control of all operations and maintenance of operator proficiency for technologically challenging applications. 2. Outreach &Education. To provide informal tutorials, formal courses, symposia, and web-based information with the goals of increasing our user base through enhanced awareness of flow cytometry and introducing established users to newer technologies and applications. 3. Development. To develop new applications in response to users'needs and to take full advantage of equipment capabilities, through discussions with users, participation in international flow cytometry meetings, and inclusion of knowledgeable core users on our Advisory Committee. To keep pace with research needs of the RDCC investigators, we have expanded the capacity of the CFCC and introduced new equipment and technologies. Continued development of innovative applications is enhanced by the depth of expertise at UAB and external collaborations. Education is accomplished through bi-weekly Individualized Design of Experiments &Analyses Sessions (IDEAs) in which the Director/Co-Directors interact with investigators to develop protocols and applications, including integration of flow cytometry aspects in the experimental design with other Cores, including AIIC and AGTC.

Public Health Relevance

The Comprehensive Flow Cytometry Core provides instrumentation and expertise to support fundamental mechanistic studies of rheumatic diseases and autoimmunity. Furthermore, the CFCC resources support the identification of new biomarkers for disease diagnosis and the development of novel treatments.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZAR1-KM)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Alabama Birmingham
United States
Zip Code
Huo, Yongliang; Lockhart, Jonathan R; Liu, Shanrun et al. (2017) Allogeneic bone marrow transplant in the absence of cytoreductive conditioning rescues mice with ?-thalassemia major. Blood Adv 1:2421-2432
Ring, Eric K; Li, Rong; Moore, Blake P et al. (2017) Newly Characterized Murine Undifferentiated Sarcoma Models Sensitive to Virotherapy with Oncolytic HSV-1 M002. Mol Ther Oncolytics 7:27-36
Botta, Davide; Fuller, Michael J; Marquez-Lago, Tatiana T et al. (2017) Dynamic regulation of T follicular regulatory cell responses by interleukin 2 during influenza infection. Nat Immunol 18:1249-1260
Nasti, Tahseen H; Cochran, J Barry; Vachhani, Raj V et al. (2017) IL-23 Inhibits Melanoma Development by Augmenting DNA Repair and Modulating T Cell Subpopulations. J Immunol 198:950-961
Pham-Hua, Dana; Padgett, Lindsey E; Xue, Bing et al. (2017) Islet encapsulation with polyphenol coatings decreases pro-inflammatory chemokine synthesis and T cell trafficking. Biomaterials 128:19-32
DeRamus, Marci L; Stacks, Delores A; Zhang, Youwen et al. (2017) GARP2 accelerates retinal degeneration in rod cGMP-gated cation channel ?-subunit knockout mice. Sci Rep 7:42545
Seleme, Maria C; Kosmac, Kate; Jonjic, Stipan et al. (2017) Tumor Necrosis Factor Alpha-Induced Recruitment of Inflammatory Mononuclear Cells Leads to Inflammation and Altered Brain Development in Murine Cytomegalovirus-Infected Newborn Mice. J Virol 91:
Gibson, Sara A; Yang, Wei; Yan, Zhaoqi et al. (2017) Protein Kinase CK2 Controls the Fate between Th17 Cell and Regulatory T Cell Differentiation. J Immunol 198:4244-4254
Robinson, Tanya O; Zhang, Mingce; Ochsenbauer, Christina et al. (2017) CD4 regulatory T cells augment HIV-1 expression of polarized M1 and M2 monocyte derived macrophages. Virology 504:79-87
Seu, Lillian; Tidwell, Christopher; Timares, Laura et al. (2017) CD151 Expression Is Associated with a Hyperproliferative T Cell Phenotype. J Immunol 199:3336-3347

Showing the most recent 10 out of 305 publications