The mission of the University of Pennsylvania Molecular Profiling Core is to provide quality services for molecular biology assays that are conducted with highly parallel or high-throughput technologies. These services include assistance with experimental design and resource assessment, sample preparation, assay performance, and data management and analysis. Professional laboratory technologists and bioinformaticists working as a team throughout an investigator's project provide these resources. The Molecular Profiling Core enables a wide variety of researchers to observe global nucleic acid patterns, including expression levels of all RNA transcripts in a sample, genetic variability throughout the genomic DNA sequence of an individual or population, and epigenetic modifications across the genome. These patterns, whether genome-wide or targeted to a specific set of markers, can be compared between control and treated/affected cell types in experiments that range from cell cultures to diagnostic or prognostic patient samples.
The Specific Aims are:
Aim 1 : To provide guidance and training on the capabilities, advantages, and disadvantages of various genomics protocols and analyses for musculoskeletal research through formal educational enrichment programs and one-on-one interactions.
Aim 2 : To provide expertise and service for whole-genome and targeted RNA profiling assays of musculoskeletal tissues.
Aim 3 : To provide expertise and service for whole-genome and targeted DNA profiling assays of musculoskeletal tissues.
Aim 4 : To provide bioinformatics services and training appropriate for analyzing the data produced in Aims 2 and 3.
Aim 5 : To provide funding for development of new assays, projects and collaborations and to facilitate development of preliminary and/or feasibility data for investigators.
Successful completion of these aims will significantly enhance the environment and the capabilities of researchers at the University of Pennsylvania, leading to new approaches to address musculoskeletal disorders and new collaborations between Center faculty who may have not previously included genomics or other molecular profiling approaches in their musculoskeletal research programs.
|VanBelzen, D Jake; Malik, Alock S; Henthorn, Paula S et al. (2017) Mechanism of Deletion Removing All Dystrophin Exons in a Canine Model for DMD Implicates Concerted Evolution of X Chromosome Pseudogenes. Mol Ther Methods Clin Dev 4:62-71|
|Robinson, Kelsey A; Sun, Mei; Barnum, Carrie E et al. (2017) Decorin and biglycan are necessary for maintaining collagen fibril structure, fiber realignment, and mechanical properties of mature tendons. Matrix Biol 64:81-93|
|Li, Qing; Qu, Feini; Han, Biao et al. (2017) Micromechanical anisotropy and heterogeneity of the meniscus extracellular matrix. Acta Biomater 54:356-366|
|Szczesny, Spencer E; Driscoll, Tristan P; Tseng, Hsiao-Yun et al. (2017) Crimped Nanofibrous Biomaterials Mimic Microstructure and Mechanics of Native Tissue and Alter Strain Transfer to Cells. ACS Biomater Sci Eng 3:2869-2876|
|Chandra, Abhishek; Lin, Tiao; Young, Tiffany et al. (2017) Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone-Forming Cells and Their Progenitors Through Distinct Mechanisms. J Bone Miner Res 32:360-372|
|Freedman, Benjamin R; Salka, Nabeel S; Morris, Tyler R et al. (2017) Temporal Healing of Achilles Tendons After Injury in Rodents Depends on Surgical Treatment and Activity. J Am Acad Orthop Surg 25:635-647|
|Johnston, Jessica M; Connizzo, Brianne K; Shetye, Snehal S et al. (2017) Collagen V haploinsufficiency in a murine model of classic Ehlers-Danlos syndrome is associated with deficient structural and mechanical healing in tendons. J Orthop Res 35:2707-2715|
|de Bakker, Chantal Mj; Altman-Singles, Allison R; Li, Yihan et al. (2017) Adaptations in the Microarchitecture and Load Distribution of Maternal Cortical and Trabecular Bone in Response to Multiple Reproductive Cycles in Rats. J Bone Miner Res 32:1014-1026|
|Martin, John T; Kim, Dong Hwa; Milby, Andrew H et al. (2017) In vivo performance of an acellular disc-like angle ply structure (DAPS) for total disc replacement in a small animal model. J Orthop Res 35:23-31|
|Uchibe, Kenta; Son, Jiyeon; Larmour, Colleen et al. (2017) Genetic and pharmacological inhibition of retinoic acid receptor ? function promotes endochondral bone formation. J Orthop Res 35:1096-1105|
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