One of the most basic needs of investigators studying skin disease is the ability to use tissue sections to analyze the pathological changes at the tissue and cellular level. This analysis requires access to specialized equipment for tissue processing and to highly trained personnel, neither of which can be afforded by most research laboratories. Furthermore, since qualitative and quantitative evaluation of skin and skin appendages requires precise and reproducible orientation of skin sections, histology services not specialized in skin generally produce skin sections of substandard quality. Therefore, the objective of the Morphology and Phenotyping Core is to provide investigators with the tools and expertise to process and analyze skin samples. The Core owns the equipment necessary to process and section skin samples and employs a histology technician trained in processing skin samples. Thus, the Core is able to provide investigators with high quality processing and sectioning of skin samples as well as with the preparation of basic histological stains. Whereas histological analysis of skin samples represents the first step in analyzing skin phenotypes, subsequent analyses include immunostaining for various markers of skin proliferation and differentiation. The Core possesses a wide variety of custom-made antibodies that recognize such markers and will provide investigators with immunostaining services using these antibodies. The Core will also offer in situ hybridization services to allow investigators to determine transcript expression patterns of their gene of interest. Finally, the Core will offer consultation services including assistance with harvesting skin samples, analyzing histological stains, analyzing antibody staining results, and analyzing in situ hybridization results.

Public Health Relevance

The Morphology and Phenotyping Core will enable UCD-SDRC members to analyze how skin structure and function are affected in various human skin diseases and in mouse models designed to mimic these disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR057212-05
Application #
8519058
Study Section
Special Emphasis Panel (ZAR1-KM-D)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$149,881
Indirect Cost
$51,919
Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Mukherjee, Nabanita; Reuland, Steven N; Lu, Yan et al. (2015) Combining a BCL2 inhibitor with the retinoid derivative fenretinide targets melanoma cells including melanoma initiating cells. J Invest Dermatol 135:842-50
O'Shea, Charlene; Fitzpatrick, James E; Koch, Peter J (2014) Desmosomal defects in acantholytic squamous cell carcinomas. J Cutan Pathol 41:873-9
Luo, Yuchun; Cai, Xiangna; Liu, Sucai et al. (2014) Suppression of antigen-specific adaptive immunity by IL-37 via induction of tolerogenic dendritic cells. Proc Natl Acad Sci U S A 111:15178-83
BarĂ³n, Anna E; Asdigian, Nancy L; Gonzalez, Victoria et al. (2014) Interactions between ultraviolet light and MC1R and OCA2 variants are determinants of childhood nevus and freckle phenotypes. Cancer Epidemiol Biomarkers Prev 23:2829-39
Koch, Peter J; Dinella, Jason; Fete, Mary et al. (2014) Modeling AEC-New approaches to study rare genetic disorders. Am J Med Genet A 164A:2443-54
Kogut, Igor; Roop, Dennis R; Bilousova, Ganna (2014) Differentiation of human induced pluripotent stem cells into a keratinocyte lineage. Methods Mol Biol 1195:1-12
Bilousova, Ganna; Roop, Dennis R (2014) Induced pluripotent stem cells in dermatology: potentials, advances, and limitations. Cold Spring Harb Perspect Med 4:a015164
Riemondy, Kent; Hoefert, Jaimee E; Yi, Rui (2014) Not miR-ly micromanagers: the functions and regulatory networks of microRNAs in mammalian skin. Wiley Interdiscip Rev RNA 5:849-65
Deng, Hui; Li, Fulun; Li, Hong et al. (2014) CtBP1 overexpression in keratinocytes perturbs skin homeostasis. J Invest Dermatol 134:1323-31
Dinella, Jason; Koster, Maranke I; Koch, Peter J (2014) Use of induced pluripotent stem cells in dermatological research. J Invest Dermatol 134:e23

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