The Pathology Core will be widely used by cutaneous investigators to aid in understanding function. This Core will provide service, instruction and interpretation. Since light microscopy and specifically routine histology and immunohistochemistry were indicated to be of the greatest general use to the SDRC members, service will be in the form of tissue processing, sectioning and staining to elucidate the morphology of specific epithelia. Investigators will be able to detect expression of genes at the message and protein levels through the use of in situ hybridization and immunohistochemical techniques, respectively. Another unique feature of the Pathology Core highly requested by SDRC users is access to a library of epithelia from various laboratory species, and the Core will include a tissue repository. Service will be in the form of expertise and instruction in more highly specialized morphological services such as laser capture microdissection, novel antibody evaluation and computer-assisted image analysis. Interpretation will help investigators evaluate whether phenotypic changes seen in epithelia from their engineered mice are different from those of wild type mice. This feature of the Core draws on the expertise that the Director and co-Directors have in investigating numerous stratified squamous epithelia and their appendages. We anticipate, based on our experience that the Pathology core will provide services, training and interpretative abilities that exceed the capabilities of the individual investigators and thus will result in the strengthening and the development of new collaborative ventures in epithelial biology.
Morphological characterization of tissues has been a one of the primary means of understanding function. The need for this these types of analyses have become even more important with the advancement in production of genetically engineered mice, which require detailed morphological descriptions of their unique phenotypes. The Pathology Core will provide SDRC members with a variety of morphological techniques and interpretative expertise to facilitate their studies. Thus collaborations among epithelial biologists at Northwestern University will be enhanced and expanded.
|Ziolo, Kevin J; Jeong, Hee-Gon; Kwak, Jayme S et al. (2014) Vibrio vulnificus biotype 3 multifunctional autoprocessing RTX toxin is an adenylate cyclase toxin essential for virulence in mice. Infect Immun 82:2148-57|
|Johnson, Jodi L; Koetsier, Jennifer L; Sirico, Anna et al. (2014) The desmosomal protein desmoglein 1 aids recovery of epidermal differentiation after acute UV light exposure. J Invest Dermatol 134:2154-62|
|Khare, Sonal; Ratsimandresy, Rojo A; de Almeida, Lúcia et al. (2014) The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses. Nat Immunol 15:343-53|
|Werner, Michael E; Mitchell, Jennifer W; Putzbach, William et al. (2014) Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1. J Cell Biol 206:367-76|
|Bhattacharyya, Swati; Tamaki, Zenshiro; Wang, Wenxia et al. (2014) FibronectinEDA promotes chronic cutaneous fibrosis through Toll-like receptor signaling. Sci Transl Med 6:232ra50|
|Hattori, Yoshiaki; Falgout, Leo; Lee, Woosik et al. (2014) Multifunctional skin-like electronics for quantitative, clinical monitoring of cutaneous wound healing. Adv Healthc Mater 3:1597-607|
|Koetsier, Jennifer L; Amargo, Evangeline V; Todorovic, Viktor et al. (2014) Plakophilin 2 affects cell migration by modulating focal adhesion dynamics and integrin protein expression. J Invest Dermatol 134:112-22|
|Todorovic, Viktor; Koetsier, Jennifer L; Godsel, Lisa M et al. (2014) Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64|
|Heffern, Marie C; Velasco, Pauline T; Matosziuk, Lauren M et al. (2014) Modulation of amyloid-? aggregation by histidine-coordinating Cobalt(III) Schiff base complexes. Chembiochem 15:1584-9|
|Robinson, June K; Gaber, Rikki; Hultgren, Brittney et al. (2014) Skin self-examination education for early detection of melanoma: a randomized controlled trial of Internet, workbook, and in-person interventions. J Med Internet Res 16:e7|
Showing the most recent 10 out of 38 publications