The Pathology Core will be widely used by cutaneous investigators to aid in understanding function. This Core will provide service, instruction and interpretation. Since light microscopy and specifically routine histology and immunohistochemistry were indicated to be of the greatest general use to the SDRC members, service will be in the form of tissue processing, sectioning and staining to elucidate the morphology of specific epithelia. Investigators will be able to detect expression of genes at the message and protein levels through the use of in situ hybridization and immunohistochemical techniques, respectively. Another unique feature of the Pathology Core highly requested by SDRC users is access to a library of epithelia from various laboratory species, and the Core will include a tissue repository. Service will be in the form of expertise and instruction in more highly specialized morphological services such as laser capture microdissection, novel antibody evaluation and computer-assisted image analysis. Interpretation will help investigators evaluate whether phenotypic changes seen in epithelia from their engineered mice are different from those of wild type mice. This feature of the Core draws on the expertise that the Director and co-Directors have in investigating numerous stratified squamous epithelia and their appendages. We anticipate, based on our experience that the Pathology core will provide services, training and interpretative abilities that exceed the capabilities of the individual investigators and thus will result in the strengthening and the development of new collaborative ventures in epithelial biology.

Public Health Relevance

Morphological characterization of tissues has been a one of the primary means of understanding function. The need for this these types of analyses have become even more important with the advancement in production of genetically engineered mice, which require detailed morphological descriptions of their unique phenotypes. The Pathology Core will provide SDRC members with a variety of morphological techniques and interpretative expertise to facilitate their studies. Thus collaborations among epithelial biologists at Northwestern University will be enhanced and expanded.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZAR1-KM-D)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Northwestern University at Chicago
United States
Zip Code
Kishibe, Mari; Griffin, Tina M; Goslawski, Melissa et al. (2018) Topical nicotinic receptor activation improves wound bacterial infection outcomes and TLR2-mediated inflammation in diabetic mouse wounds. Wound Repair Regen 26:403-412
Kam, Chen Yuan; Dubash, Adi D; Magistrati, Elisa et al. (2018) Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43. J Cell Biol 217:3219-3235
North, Jeffrey P; Golovato, Justin; Vaske, Charles J et al. (2018) Cell of origin and mutation pattern define three clinically distinct classes of sebaceous carcinoma. Nat Commun 9:1894
Rübsam, Matthias; Broussard, Joshua A; Wickström, Sara A et al. (2018) Adherens Junctions and Desmosomes Coordinate Mechanics and Signaling to Orchestrate Tissue Morphogenesis and Function: An Evolutionary Perspective. Cold Spring Harb Perspect Biol 10:
Chu, Lan H; Indramohan, Mohanalaxmi; Ratsimandresy, Rojo A et al. (2018) The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun 9:996
Tsoi, Lam C; Yang, Jingjing; Liang, Yun et al. (2018) Transcriptional determinants of individualized inflammatory responses at anatomically separate sites. J Allergy Clin Immunol 141:805-808
Kaplan, Nihal; Ventrella, Rosa; Peng, Han et al. (2018) EphA2/Ephrin-A1 Mediate Corneal Epithelial Cell Compartmentalization via ADAM10 Regulation of EGFR Signaling. Invest Ophthalmol Vis Sci 59:393-406
Nekrasova, Oxana; Harmon, Robert M; Broussard, Joshua A et al. (2018) Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat Commun 9:1053
Ratsimandresy, Rojo A; Chu, Lan H; Khare, Sonal et al. (2017) The PYRIN domain-only protein POP2 inhibits inflammasome priming and activation. Nat Commun 8:15556
Bagchi, Sreya; He, Ying; Zhang, Hong et al. (2017) CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice. J Clin Invest 127:2339-2352

Showing the most recent 10 out of 102 publications