The Muscle Genomics/Bioinformatics Core is designed to enable muscle researchers to access state of the art genomic tools and use sophisticated data processing tools within the core. The core will be available to inestigators to provide advice and guidance on the use of microarray and massively parallel sequencing tools for genomic assessment. The core will provide high density genome wide SNP analysis for genome-wide association studies on large datasets and linkage analysis within small families. Second the core provides genome wide assessment of RNA abundance. Third the core provides genome -scale assessment of alternative splicing. Fourth, the core provides analytical support for the.process of microarray gene expression data, microarray based SNP data, and massively parallel sequencing data.
The Aims of the Core are Aim 1: To enable researchers to perform gene expression analysis, alternative splicing analysis, copy number analysis, mutation analysis, SNP analysis and methylation analysis on a genome scale;
Aim 2 : To provide analytical advice and guidance;
Aim 3 : To provide researchers access to large microarray datasets and training in genomic analysis. This core facility will be a valuable resource to Center Investigators as well as to the greater dystrophy community by facilitating rapid genomic analyses. This Core is integral to the overall functioning of the Center.

Public Health Relevance

This core facility will be a valuable resource to Center Investigators as well as to the greater dystrophy community by facilitating genomic analysis of muscle tissues/cell lines. These studies augment specific cell biology experimentation ongoing by Center members

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR057230-05
Application #
8459891
Study Section
Special Emphasis Panel (ZAR1-CHW-G)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
5
Fiscal Year
2013
Total Cost
$185,006
Indirect Cost
$68,371
Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Aliotta, Eric; Moulin, Kévin; Magrath, Patrick et al. (2018) Quantifying precision in cardiac diffusion tensor imaging with second-order motion-compensated convex optimized diffusion encoding. Magn Reson Med 80:1074-1087
Hicks, Michael R; Hiserodt, Julia; Paras, Katrina et al. (2018) ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs. Nat Cell Biol 20:46-57
Armstrong, Tess; Dregely, Isabel; Stemmer, Alto et al. (2018) Free-breathing liver fat quantification using a multiecho 3D stack-of-radial technique. Magn Reson Med 79:370-382
Wang, Richard T; Barthelemy, Florian; Martin, Ann S et al. (2018) DMD genotype correlations from the Duchenne Registry: Endogenous exon skipping is a factor in prolonged ambulation for individuals with a defined mutation subtype. Hum Mutat 39:1193-1202
Wang, Derek W; Mokhonova, Ekaterina I; Kendall, Genevieve C et al. (2018) Repurposing Dantrolene for Long-Term Combination Therapy to Potentiate Antisense-Mediated DMD Exon Skipping in the mdx Mouse. Mol Ther Nucleic Acids 11:180-191
Kramerova, Irina; Torres, Jorge A; Eskin, Ascia et al. (2018) Calpain 3 and CaMKII? signaling are required to induce HSP70 necessary for adaptive muscle growth after atrophy. Hum Mol Genet 27:1642-1653
Gibbs, Elizabeth M; Crosbie-Watson, Rachelle H (2017) A Simple and Low-cost Assay for Measuring Ambulation in Mouse Models of Muscular Dystrophy. J Vis Exp :
Peter, Angela K; Miller, Gaynor; Capote, Joana et al. (2017) Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F. Skelet Muscle 7:11
Young, Courtney S; Mokhonova, Ekaterina; Quinonez, Marbella et al. (2017) Creation of a Novel Humanized Dystrophic Mouse Model of Duchenne Muscular Dystrophy and Application of a CRISPR/Cas9 Gene Editing Therapy. J Neuromuscul Dis 4:139-145
Rai, Muhammad Farooq; Duan, Xin; Quirk, James D et al. (2017) Post-Traumatic Osteoarthritis in Mice Following Mechanical Injury to the Synovial Joint. Sci Rep 7:45223

Showing the most recent 10 out of 71 publications