Form and function are intimately interrelated. However, the histological analyses of our target tissues (bone, cartilage, tendon, ligament, muscle) provide specialized challenges not often encountered when conducting these techniques on non-skeletal tissues. For example, due to the extensive extracellular matrix, these tissues can be very difficult to prepare and section, and bone presents a particular challenge due to mineralization. A specialized knowledge of the stages of development, and limb and joint morphology is necessary for the analysis of skeletal tissues. In addition to morphology, the localization of proteins and mRNA in mouse models of musculoskeletal diseases is a vital component in the analysis of the molecular mechanism of protein function, the response to injury, and disease. The overall objective of the In situ Molecular Analysis Core is to provide histological services for the identification and analysis of molecular phenotypes of our target tissues, bone, cartilage, muscle, tendon and ligament, in developing and adult mice. The services will include preparation of tissues and tissue sections;a reagent bank for histology, immunohistochemistry and in situ hybridization;and training in the techniques of in situ hybridization to mRNA, immunohistochemistry, deconvolution microscopy, and histomorphometry. Reagents for the analysis of mouse, and in some cases human, tissues will be available. We expect that the findings from mouse models will be extended to human disease and injury. The Director/Co-Directors and collaborating investigators will provide consultation and advice on analysis and interpretation of the target tissue phenotypes. Deconvolution microscopic analysis and bone histomorphometry will provide the opportunity to utilize sophisticated techniques and quantitative analysis not currently available to all investigators. The strength of this Core lies in the specific techniques developed for adult mice, sectioning delicate early healing tissue, and properly aligning blocks for sectioning of ligament and fracture healing, as well as the expertise in tissue staining by a wide variety of histochemical and molecular techniques. All aspects of the core provide collaboration, training, and education, which will strengthen ties among the research base and enhance the quality of the science performed. Thus, not only will this Core be cost-effective, it will allow scientists to explore skeletal phenotypes that they could not in any other way investigate. This core will complement the specific functional analyses proposed in Core B and the mouse models of Core D.
The In Situ Molecular Analysis Core (C) will allow our scientists to maximize the amount of data derived from animal models of osteoporosis, arthritis, musculoskeletal injury, cancer metastasis to bone, and other musculoskeletal diseases by providing access to a number of sophisticated techniques and reagents.
|Oladipupo, Sunday S; Smith, Craig; Santeford, Andrea et al. (2014) Endothelial cell FGF signaling is required for injury response but not for vascular homeostasis. Proc Natl Acad Sci U S A 111:13379-84|
|McAlinden, Audrey; Traeger, Geoffrey; Hansen, Uwe et al. (2014) Molecular properties and fibril ultrastructure of types II and XI collagens in cartilage of mice expressing exclusively the *1(IIA) collagen isoform. Matrix Biol 34:105-13|
|McAlinden, Audrey (2014) Alternative splicing of type II procollagen: IIB or not IIB? Connect Tissue Res 55:165-76|
|Tomlinson, Ryan E; Schmieder, Anne H; Quirk, James D et al. (2014) Antagonizing the ?v ?3 integrin inhibits angiogenesis and impairs woven but not lamellar bone formation induced by mechanical loading. J Bone Miner Res 29:1970-80|
|Holguin, Nilsson; Aguilar, Rhiannon; Harland, Robin A et al. (2014) The aging mouse partially models the aging human spine: lumbar and coccygeal disc height, composition, mechanical properties, and Wnt signaling in young and old mice. J Appl Physiol (1985) 116:1551-60|
|Rai, Muhammad Farooq; Patra, Debabrata; Sandell, Linda J et al. (2014) Relationship of gene expression in the injured human meniscus to body mass index: a biologic connection between obesity and osteoarthritis. Arthritis Rheumatol 66:2152-64|
|Tatara, Alexander M; Lipner, Justin H; Das, Rosalina et al. (2014) The role of muscle loading on bone (Re)modeling at the developing enthesis. PLoS One 9:e97375|
|Alspach, Elise; Flanagan, Kevin C; Luo, Xianmin et al. (2014) p38MAPK plays a crucial role in stromal-mediated tumorigenesis. Cancer Discov 4:716-29|
|Sato, Eugene J; Killian, Megan L; Choi, Anthony J et al. (2014) Skeletal muscle fibrosis and stiffness increase after rotator cuff tendon injury and neuromuscular compromise in a rat model. J Orthop Res 32:1111-6|
|Hering, Thomas M; Wirthlin, Louisa; Ravindran, Soumya et al. (2014) Changes in type II procollagen isoform expression during chondrogenesis by disruption of an alternative 5' splice site within Col2a1 exon 2. Matrix Biol 36:51-63|
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