Skin samples from patients with systemic sclerosis (SSc) are not widely available to investigators studying pathogenesis. To derive the greatest value from these samples it is important that they are associated with clinical data, in particular the modified Rodnan skin score (MRSS), but also ideally other organ involvement The Boston University Medical Center Scleroderma Program under the leadership of Dr. Lafyatis and through NIH U01 funding has collected and is continuing to collect such well-characterized biopsies. In addition in many cases in patients with early diffuse SSc biopsies are being collected every 6 months, so that the evolution of the disease and the relationship to changes in protein staining in the skin tissues can be compared. Dr. Bhawan, Dermatopathologist and Director of the BUMC Dermatopathology Laboratory, will direct the Skin Pathology Core (Core B). The Core will process these and other biopsies provided by Core Center Investigators into 8-sample tissue blocks containing either 6 different SSc and 2 healthy control biopsies ("Discovery slides"), 6 temporally evolving skin changes in individual patients and 2 healthy control biopsies ("Disease Progression slides"), or 2 SSc and 6 other diseases including wound tissue, psoriasis, keloid and nephrogenic systemic fibrosis (Specificity slides). Murine skin will be collected from murine models of SSc: cGVHD, bleomycin, Tsk and other SSc models, as well as control mice for similar multi-tissue slide preparations. The Core will assist Investigators or stain these slides in the Core as the Investigator requests with proteins under study for new pathological processes implicated in the vasculopathy, fibrotic or immune response in SSc and murine SSc model skin. Core B will also evaluate histochemical or immunohistochemical staining intensity using quantitative (image analysis) or semi-quantitative methods. Sample staining will be correlated with MRSS and other clinical parameters, and past staining patterns in slides from the same block can also be compared. These slides will provide an unparalleled resource for discovery of pathogenic mechanisms in skin pathogenesis of SSc.

Public Health Relevance

SSc is a devastating rheumatic condition often leading to death. Because the disease is uncommon, it has been difficult to organize resources and obtain adequate sample numbers to understand disease pathogenesis. This Core will provide a mechanism for obtaining skin samples from large numbers of patients for evaluation by histochemistry and immunohistochemistry.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR061271-04
Application #
8734890
Study Section
Special Emphasis Panel (ZAR1-MLB)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
4
Fiscal Year
2014
Total Cost
$185,452
Indirect Cost
$92,365
Name
Boston University
Department
Type
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
Fan, Ming-Hui; Feghali-Bostwick, Carol A; Silver, Richard M (2014) Update on scleroderma-associated interstitial lung disease. Curr Opin Rheumatol 26:630-6
Farina, Antonella; Cirone, Mara; York, Michael et al. (2014) Epstein-Barr virus infection induces aberrant TLR activation pathway and fibroblast-myofibroblast conversion in scleroderma. J Invest Dermatol 134:954-64
Arron, Sarah T; Dimon, Michelle T; Li, Zhenghui et al. (2014) High Rhodotorula sequences in skin transcriptome of patients with diffuse systemic sclerosis. J Invest Dermatol 134:2138-45
Assassi, Shervin; Wu, Minghua; Tan, Filemon K et al. (2013) Skin gene expression correlates of severity of interstitial lung disease in systemic sclerosis. Arthritis Rheum 65:2917-27