The Phenotyping Core (Core B) is designed to evaluate the functional properties of muscles, joints, and whole animals in animal models created by the various Center investigators or directly in human muscle. The Phenotyping Core will interface with the High Throughput Cell Sorting Core and the Imaging Core by helping Center investigators identify the appropriate functional evaluation of muscle, with special consideration of the specific manipulation created by the Center investigator. The quantitative functional evaluation conducted by the Phenotyping Core will often be combined with structural data obtained from the Imaging Core thereby providing possible mechanistic explanations for any functional effects that are found. Thus, the Phenotyping Core will provide accessibility to sophisticated physiological and biomechanical testing capabilities to all Center investigators.
The Specific Aims of the Phenotyping Core are: 1) To provide training and technical assistance in skeletal muscle identification, dissection and mounting for isolated contractile and metabolic studies of isolated muscles. 2) To provide training and technical assistance in developing specialized """"""""jigs"""""""" and interfaces to measure joint or muscle torque, or to conduct whole animal funcfional measurements. 3) To provide technical assistance with snap-freezing and routine histological processing of skeletal muscle including hematoxylin and eosin, immunohistochemistry, quantitative morphometry, and qualitafive morphology. 4) To provide training and technical assistance in measuring various aspects of metabolism either in vivo of ex vivo in muscle.
One of the great strengths of the Phenotyping Core are the multiple scale levels on which function can be assessed including the whole animal (on treadmills), whole joint (via torque motors), whole muscle (using in vitro testing) and isolated single fibers (also using in vitro testing). Thus, this Core plays an essential role in the proposed Center's long-term goal to achieve a comprehensive understanding of multi-scale structure function relationships in skeletal muscles. Since most of the devices in the core are highly specialized, they are generally unavailable to Center investigators, except through mechanisms such as the P30. This core thus fills a significant void in the local San Diego muscle research community.
|Sala, David; Sacco, Alessandra (2016) Signal transducer and activator of transcription 3 signaling as a potential target to treat muscle wasting diseases. Curr Opin Clin Nutr Metab Care 19:171-6|
|Malecova, Barbora; Dall'Agnese, Alessandra; Madaro, Luca et al. (2016) TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells. Elife 5:|
|Tierney, Matthew T; Sacco, Alessandra (2016) Satellite Cell Heterogeneity in Skeletal Muscle Homeostasis. Trends Cell Biol 26:434-44|
|Toto, Paula Coutinho; Puri, Pier Lorenzo; Albini, Sonia (2016) SWI/SNF-directed stem cell lineage specification: dynamic composition regulates specific stages of skeletal myogenesis. Cell Mol Life Sci 73:3887-96|
|Thompson, William R; Scott, Alexander; Loghmani, M Terry et al. (2016) Understanding Mechanobiology: Physical Therapists as a Force in Mechanotherapy and Musculoskeletal Regenerative Rehabilitation. Phys Ther 96:560-9|
|Cho, Yoshitake; Hazen, Bethany C; Gandra, Paulo G et al. (2016) Perm1 enhances mitochondrial biogenesis, oxidative capacity, and fatigue resistance in adult skeletal muscle. FASEB J 30:674-87|
|Tierney, Matthew; Garcia, Christina; Bancone, Matthew et al. (2016) Innervation of dystrophic muscle after muscle stem cell therapy. Muscle Nerve 54:763-8|
|Tierney, Matthew Timothy; Gromova, Anastasia; Sesillo, Francesca Boscolo et al. (2016) Autonomous Extracellular Matrix Remodeling Controls a Progressive Adaptation in Muscle Stem Cell Regenerative Capacity during Development. Cell Rep 14:1940-52|
|Kinney, Matthew C; Dayanidhi, Sudarshan; Dykstra, Peter B et al. (2016) Reduced skeletal muscle satellite cell number alters muscle morphology after chronic stretch but allows limited serial sarcomere addition. Muscle Nerve :|
|Fiacco, E; Castagnetti, F; Bianconi, V et al. (2016) Autophagy regulates satellite cell ability to regenerate normal and dystrophic muscles. Cell Death Differ 23:1839-1849|
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