Since its establishment in November 2004, the Sidney Kimmel Comprehensive Cancer Center (SKCCC) Microarray Core has provided services with affordable rates and flexibility in a timely manner to Cancer Center members across the Hopkins Medical Campus. The Core operates such that it supplies platforms not primarily offered by other array Cores throughout the School of Medicine and it is closely linked, for high level computational needs of users, to the new Bioinformatics Core in the Cancer Center. Since the last renewal, the Core has added to its full Agilent expression and DNA platforms, lllumina Infinium BeadChip technology including the Infinium platform for genome wide, promoter region DNA methylation. Thus, using both Agilent and lllumina technologies, the Core now offers assays for gene expression, micro RNA expression, DNA copy number variation, ChlP-on-chip, and genome-wide DNA methylation profiling. SNP analysis is also possible with the Core's current platforms. The probe density on the arrays and level of sample throughput have been increased significantly since the last renewal to efficiently meet the increased research needs of Cancer Center members. The Core's services also include DNA and RNA extraction from submitted samples, quality assessment of all submitted RNA and DNA samples, bisulfate treatment and quality control assessment of DNA for the Infinium methylation platform, and experiment design consultation. Real time PCR confirmation of expression array data is also provided. Preliminary data analysis and help in using software for data analysis are offered. The Core participates in computational biology seminars arranged by the Bioinformatics Core and arranges presentations by Agilent, lllumina, and other array offering companies to all Cancer Center and others throughout the School of Medicine. Lay: The Microarray Core provides different DNA microarray solutions to meet the needs of genomic research as well as related services including sample isolation and software training for data analysis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA006973-51
Application #
8661009
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
51
Fiscal Year
2014
Total Cost
$201,928
Indirect Cost
$77,534
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Isenberg, Sarina R; Lu, Chunhua; McQuade, John et al. (2017) Impact of a New Palliative Care Program on Health System Finances: An Analysis of the Palliative Care Program Inpatient Unit and Consultations at Johns Hopkins Medical Institutions. J Oncol Pract 13:e421-e430
Husain, Hatim; Velculescu, Victor E (2017) Cancer DNA in the Circulation: The Liquid Biopsy. JAMA 318:1272-1274
Holdhoff, Matthias; Cairncross, Gregory J; Kollmeyer, Thomas M et al. (2017) Genetic landscape of extreme responders with anaplastic oligodendroglioma. Oncotarget 8:35523-35531
Oh, Min-Hee; Collins, Samuel L; Sun, Im-Hong et al. (2017) mTORC2 Signaling Selectively Regulates the Generation and Function of Tissue-Resident Peritoneal Macrophages. Cell Rep 20:2439-2454
Klein, Orly R; Buddenbaum, Jessica; Tucker, Noah et al. (2017) Nonmyeloablative Haploidentical Bone Marrow Transplantation with Post-Transplantation Cyclophosphamide for Pediatric and Young Adult Patients with High-Risk Hematologic Malignancies. Biol Blood Marrow Transplant 23:325-332
Johnson 3rd, Burles A; Yarchoan, Mark; Lee, Valerie et al. (2017) Strategies for Increasing Pancreatic Tumor Immunogenicity. Clin Cancer Res 23:1656-1669
Antonarakis, Emmanuel S; Lu, Changxue; Luber, Brandon et al. (2017) Clinical Significance of Androgen Receptor Splice Variant-7 mRNA Detection in Circulating Tumor Cells of Men With Metastatic Castration-Resistant Prostate Cancer Treated With First- and Second-Line Abiraterone and Enzalutamide. J Clin Oncol 35:2149-2156
Cohen, Joshua D; Javed, Ammar A; Thoburn, Christopher et al. (2017) Combined circulating tumor DNA and protein biomarker-based liquid biopsy for the earlier detection of pancreatic cancers. Proc Natl Acad Sci U S A 114:10202-10207
McCurdy, Shannon R; Kasamon, Yvette L; Kanakry, Christopher G et al. (2017) Comparable composite endpoints after HLA-matched and HLA-haploidentical transplantation with post-transplantation cyclophosphamide. Haematologica 102:391-400
Isella, Claudio; Brundu, Francesco; Bellomo, Sara E et al. (2017) Selective analysis of cancer-cell intrinsic transcriptional traits defines novel clinically relevant subtypes of colorectal cancer. Nat Commun 8:15107

Showing the most recent 10 out of 2207 publications