The main objective of the Molecular Cytology Core Facility (MCCF) is to provide to users state-of-the-arttechnologies in a multi-user environment, for preparation of tissue samples, for detection, precise localizationand analysis of the expression of molecules with important cell functions during development and in cancer.The technologies include in situ localization of mRNA and proteins, cell proliferation, stem cell potential anddifferentiation, apoptosis and senescence, angiogenesis and hypoxia, histological evaluation and molecularin situ characterization of phenotypes of knock- out, transgenic mice and human tumors. In addition to thetraining and assistance provided to the researchers in the execution of these technologies, automatedexperiments for in situ molecular detection are carried at the MCCF. The optical microscopes [wide field,laser scanning (point scanning, spinning disc and multiphoton)] are efficiently used by researchers for imageacquisition, including live imaging. Experienced MCCF staff is instrumental in assisting users withstereological analysis, co-localization studies and 3D reconstructions.Specific services provided are: tissue processing and sample preparation; execution of methods for in situmolecular detection; application of a wide range of optical microscopy techniques in conjunction with imageacquisition and analysis.
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