The Molecular Cytogenetics Core provides MSKCC investigators with effective chromosome-based analysis for both human and research animal cells and provides valuable support to investigators focused on understanding chromosomal instability in cancer, documentation of cell line identity and chromosomal integrity following genetic manipulations. The Molecular Cytogenetics Core processes samples from primary cells, cell lines, or archival tissue. The Core performs chromosome analysis on research samples, using conventional cytogenetics (chromosome banding and karyotyping) and molecular cytogenetics procedures based on fluorescence in situ hybridization (FISH), including Spectral Karyotyping. Staff assist investigators in designing the most appropriate and efficient analyses for their needs and produce customized probes for specific projects. The Core has assembled a broad range of molecular cytogenetics resources, including locus-specific plasmid and BAC done stocks, and individual whole chromosome painting probes. For example, the Massague lab examined the molecular basis of the link between metastasis and chemoresistance. Cancer cells that overexpress CXCL1/2 were examined. The Core provided preparation of specific probes and performed FISH to confirm sex-mismatched mouse bone marrow engraftment (XY FISH on blood smears), and performed CXCL1/2 DNA copy analysis on tissue sections of human breast tumor and metastases (frozen, paraffin, and tissue microarray) to demonstrate copy number increase and amplification. The services and collaborative work provided by the Molecular Cytogenetics Core have supported the research of 29 investigators in the past year. During the past grant period, the work of the Core has contributed to 101 publications of researchers from 6 research programs.

Public Health Relevance

Chromosome analysis is a fundamental component of cell-based research. Tumor cells frequently have highly rearranged karyotypes and even normal cells may acquire chromosomal abnormalities after extended periods in cell culture. MSKCC investigators regularly need to examine the chromosomal status of cells in a variety of ways, from isolated DNA to whole chromosomes in live cells. The Molecular Cytogenics core provides the services and expertise to meet these needs.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA008748-48
Application #
8933514
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
2014-01-01
Project End
2018-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
48
Fiscal Year
2014
Total Cost
$215,572
Indirect Cost
$94,260
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Pak, Linda M; Gonen, Mithat; Seier, Kenneth et al. (2018) Can physician gestalt predict survival in patients with resectable pancreatic adenocarcinoma? Abdom Radiol (NY) 43:2113-2118
Thomas, Rozario; Marks, Daniel Henry; Chin, Yvette et al. (2018) Whole chromosome loss and associated breakage-fusion-bridge cycles transform mouse tetraploid cells. EMBO J 37:201-218
Shoag, Jonathan; Liu, Deli; Blattner, Mirjam et al. (2018) SPOP mutation drives prostate neoplasia without stabilizing oncogenic transcription factor ERG. J Clin Invest 128:381-386
Abreu, Carla M; Prakash, Rohit; Romanienko, Peter J et al. (2018) Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination. Nat Commun 9:3961
Katsoulakis, Evangelia; Leeman, Jonathan E; Lok, Benjamin H et al. (2018) Long-term outcomes in oral cavity squamous cell carcinoma with adjuvant and salvage radiotherapy after surgery. Laryngoscope 128:2539-2545
Huicochea Castellanos, Sandra; Corrias, Giuseppe; Ulaner, Gary A et al. (2018) Detection of recurrent pancreatic cancer: value of second-opinion interpretations of cross-sectional images by subspecialized radiologists. Abdom Radiol (NY) :
Hoseini, Sayed Shahabuddin; Guo, Hongfen; Wu, Zhihao et al. (2018) A potent tetravalent T-cell-engaging bispecific antibody against CD33 in acute myeloid leukemia. Blood Adv 2:1250-1258
Attiyeh, Marc A; Fernández-Del Castillo, Carlos; Al Efishat, Mohammad et al. (2018) Development and Validation of a Multi-institutional Preoperative Nomogram for Predicting Grade of Dysplasia in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas: A Report from The Pancreatic Surgery Consortium. Ann Surg 267:157-163
Tollinche, Luis; Tan, KaySee; Han, Austin et al. (2018) Analyzing Volatile Anesthetic Consumption by Auditing Fresh Gas Flow: An Observational Study at an Academic Hospital. Int J Anesth Anesth 5:
Tadros, Audree B; Wen, Hannah Y; Morrow, Monica (2018) Breast Cancers of Special Histologic Subtypes Are Biologically Diverse. Ann Surg Oncol 25:3158-3164

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