The primary goal of the X-ray Crystallography Core Facility is to provide access to state-of-the-art hardware, software and expertise to Cancer Center laboratories that use X-ray crystallography as a tool to address questions in their research programs. The Core provides and maintains In-house equipment and computational resources for data collection, processing and structure determination, implements a wide range of crystallographic and structure analysis software packages, participates in multi-institutional consortia for long-term regular access to synchrotron beamlines, and provides training and technical assistance to users of both the in-house and remote facilities. In addition, the Core Head collaborates with non-structural MSKCC groups on macromolecular crystallization and structure-determination projects, and also provides modeling expertise and guidance to those laboratories that can benefit from the use of available structures in the design and interpretation of experiments. Understanding the biological processes involved in tumorigenesis has increasingly benefitted from structural investigations that provide key functional and mechanistic Insights, as well as atomic-level details important for drug discovery. Many of the questions require the structure determination of large proteins, assemblies and macromolecular machines. Advances in protein expression technologies and nano-liter crystallization robots are making it possible to obtain hitherto inaccessible crystals. However, crystals of such complexes often present major experimental challenges due to small crystal size, large unit cells, poor order and limited diffraction. The X-ray Core alms to help address these limitations for MSKCC investigators by providing the latest generation in-house X-ray diffraction systems, by testing and implementing new methods, tools and software and disseminating the latest improvements, and by participating in multi-institutional consortia that not only build and operate synchrotron beamlines, but also carry out technology research and development that push the envelope of beamline capabilities. The X-Ray Crystallography Core has supported 8 investigators in the past year. During the past grant period the Core has contributed to 115 publications of researchers from 3 programs.

Public Health Relevance

Understanding the biological processes involved in tumorigenesis requires the understanding of the underlying structures of relevant proteins, genes and enzymes. The X-Ray Crystallography core provides services to MSKCC investigators interested in structure-function determinations.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA008748-48
Application #
8933521
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
2014-01-01
Project End
2018-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
48
Fiscal Year
2014
Total Cost
$271,886
Indirect Cost
$118,883
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Orlow, I; Satagopan, J M; Berwick, M et al. (2015) Genetic factors associated with naevus count and dermoscopic patterns: preliminary results from the Study of Nevi in Children (SONIC). Br J Dermatol 172:1081-9
Carey, Bryce W; Finley, Lydia W S; Cross, Justin R et al. (2015) Intracellular ?-ketoglutarate maintains the pluripotency of embryonic stem cells. Nature 518:413-6
Mosher, C E; Given, B A; Ostroff, J S (2015) Barriers to mental health service use among distressed family caregivers of lung cancer patients. Eur J Cancer Care (Engl) 24:50-9
Navi, Babak B; Reiner, Anne S; Kamel, Hooman et al. (2015) Association between incident cancer and subsequent stroke. Ann Neurol 77:291-300
Xu, Zhe; Wu, Chaochao; Xie, Fang et al. (2015) Comprehensive quantitative analysis of ovarian and breast cancer tumor peptidomes. J Proteome Res 14:422-33
Xu, Hong; Cheng, Ming; Guo, Hongfen et al. (2015) Retargeting T cells to GD2 pentasaccharide on human tumors using Bispecific humanized antibody. Cancer Immunol Res 3:266-77
Gondo, Tatsuo; Poon, Bing Ying; Matsumoto, Kazuhiro et al. (2015) Clinical role of pathological downgrading after radical prostatectomy in patients with biopsy confirmed Gleason score 3 + 4 prostate cancer. BJU Int 115:81-6
Ripley, R Taylor; McMillan, Robert R; Sima, Camelia S et al. (2014) Second primary lung cancers: smokers versus nonsmokers after resection of stage I lung adenocarcinoma. Ann Thorac Surg 98:968-74
Ye, Jiangbin; Fan, Jing; Venneti, Sriram et al. (2014) Serine catabolism regulates mitochondrial redox control during hypoxia. Cancer Discov 4:1406-17
Lu, Zhigang; Xu, Jin; Xu, Mingming et al. (2014) Morphine regulates expression of *-opioid receptor MOR-1A, an intron-retention carboxyl terminal splice variant of the *-opioid receptor (OPRM1) gene via miR-103/miR-107. Mol Pharmacol 85:368-80

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