The goal of the DNA Sequencing Shared Facility (DSSF) is to provide UAB Comprehensive Cancer Center (CCC) members with state-of-the-art automated Sanger sequencing. To this end, the specific aims are: 1. To continue to provide state-of-the-art automated DNA sequencing capabilities by maintaining dedicated Applied Biosystems Sequencers (ABI 3730XL) with capillary electrophoresis systems. 2. To continue to provide technical support and training to Cancer Center members, especially junior investigators, in DNA template preparation, primer design, cycle sequencing methodologies, and standard DNA sequence editing and analysis. To expand facility functions by providing microsatellite analyses and real-time PCR technologies as new services. To effectively interface with the Microarray Shared Facility (MSF) and the Comprehensive Genomics Shared Facility (CGSF) to assure services are complementary and not overlapping. Although originally established within the Center for AIDS Research (in 1987), CCC members now account for 70% of this facility's usage. This is because numerous laboratories within the Cancer Center are absolutely dependent on high throughput DNA sequencing for their research programs. The DSSF has ensured that CCC members have access to this service in a timely and cost-effective manner. In the last budget period, the DSSF has developed new methodologies for the automated sequencing process, trained CCC investigators in template preparation and the use of sequence analysis tools, upgraded and modernized pertinent equipment, employed automation technologies to expand capacity, and installed a Laboratory Information Management System to optimize the user interface. Since 2004, the DSSF has supported 104 CCC investigators and provided essential services for more than 175 cancer and cancer related grants and contracts. More than 458 million base pairs of primary sequence were determined, generating more than $2,710,000 in user charge-backs. Newly implemented automation increased the facility's efficiency and allowed the DSSF to reduce user charge-backs from $8 to $6 per sequencing reaction ($4 per reaction for 96 well plates).
The ability to rapidly and accurately sequence DNA is necessary for cancer-related research ranging from basic cell biology, immunology and genetics to the development of new cancer vaccines and treatments. Access to low-cost and rapid sequencing is fundamental to the success of both basic and translational science programs. Many CCC members need and depend on this service. The DNA Sequencing Shared Facility thus represents an integral component of the Comprehensive Cancer Center.
|Subramaniam, Akila; Blanchard, Christina T; Erickson, Britt K et al. (2018) Feasibility of Complete Salpingectomy Compared With Standard Postpartum Tubal Ligation at Cesarean Delivery: A Randomized Controlled Trial. Obstet Gynecol 132:20-27|
|Kasten, Benjamin B; Oliver, Patsy G; Kim, Harrison et al. (2018) 212Pb-Labeled Antibody 225.28 Targeted to Chondroitin Sulfate Proteoglycan 4 for Triple-Negative Breast Cancer Therapy in Mouse Models. Int J Mol Sci 19:|
|Stoll, Matthew L; Weiss, Pamela F; Weiss, Jennifer E et al. (2018) Age and fecal microbial strain-specific differences in patients with spondyloarthritis. Arthritis Res Ther 20:14|
|Garner, Evan F; Williams, Adele P; Stafman, Laura L et al. (2018) FTY720 Decreases Tumorigenesis in Group 3 Medulloblastoma Patient-Derived Xenografts. Sci Rep 8:6913|
|Locke, Landon W; Kothandaraman, Shankaran; Tweedle, Michael et al. (2018) Use of a leukocyte-targeted peptide probe as a potential tracer for imaging the tuberculosis granuloma. Tuberculosis (Edinb) 108:201-210|
|Fancy, Romone M; Kim, Harrison; Napier, Tiara et al. (2018) Calmodulin antagonist enhances DR5-mediated apoptotic signaling in TRA-8 resistant triple negative breast cancer cells. J Cell Biochem 119:6216-6230|
|Barrington, David A; Champion, Macie L; Boitano, Teresa K L et al. (2018) Characteristics of African American women at high-risk for ovarian cancer in the southeast: Results from a Gynecologic Cancer Risk Assessment Clinic. Gynecol Oncol 149:337-340|
|Banerjee, N Sanjib; Wang, Hsu-Kun; Beadle, James R et al. (2018) Evaluation of ODE-Bn-PMEG, an acyclic nucleoside phosphonate prodrug, as an antiviral against productive HPV infection in 3D organotypic epithelial cultures. Antiviral Res 150:164-173|
|Keene, Kimberly S; King, Tari; Hwang, E Shelley et al. (2018) Molecular determinants of post-mastectomy breast cancer recurrence. NPJ Breast Cancer 4:34|
|Kleinpeter, Alex B; Jureka, Alexander S; Falahat, Sally M et al. (2018) Structural analyses reveal the mechanism of inhibition of influenza virus NS1 by two antiviral compounds. J Biol Chem 293:14659-14668|
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