The Biomarkers Shared Resource, jointly managed by the Cancer Center and Columbia's NIEHS Center for Envoronmental Health in Northern Manhattan provides a centralized, efficient and cost-effective resource for receiving, handling and storing human samples that are collected as part of research studies in molecular epidemiology and other types of cancer-related research. It is a joint resource of the Cancer Center and Columbia's NIEHS Center for Environmental Health in Northern Manhattan. Services provided include: ? Preparation of kits for biospecimen collection ? Processing and storage of blood components, including cells and plasma ? Storage of other body fluids, including urine, sputum, and oral cells ? Isolation of DNA with quality control ? Maintenance of a sample inventory ? High to medium-throughput custom single nucleotide polymorphism genotyping Consultation is provided on all aspects of sample collection and processing for specific studies including the types of tubes needed for sample collection, shipment methods, separation of specific fractions, number of aliquots, etc. The samples processed and/or stored include blood, urine, oral cells, sputum and microscope slides of smeared cells or paraffin sections. Coded samples are received by the facility and processed into appropriate fractions (e.g., plasma, mononuclear cells, granulocytes, total white blood cells and red blood cells) and frozen in multiple aliquots in more that one freezer whenever possible. A webbased data base of the sample inventory is maintained and samples retrieved as as specified in the governing protocol. A telephone alarm system is used to ensure sample safety. Additional services include DNA isolation from buccal and white blood cells, whole genome amplification of limited DNA samples, and high throughput genotyping for single nucleotide polymorphisms in candidate genes. Samples from almost 24,000 subjects participating in 50 different studies, 43 cancer-related, are currently stored in the Biomarkers Laboratory with several new studies initiated in the current grant cycle. Future plans include expansion of patient recruitment to additional cancer sites, expansion of the available freezer space and continual updating of genotyping platforms as reliable and cost-effective new technologies become available. The operating budget is $388,000, of which we are requesting $87,730 from the CCSG.
| Mumau, Melanie D; Vanderbeck, Ashley N; Lynch, Elizabeth D et al. (2018) Identification of a Multipotent Progenitor Population in the Spleen That Is Regulated by NR4A1. J Immunol 200:1078-1087 |
| Caviglia, Jorge Matias; Yan, Jun; Jang, Myoung-Kuk et al. (2018) MicroRNA-21 and Dicer are dispensable for hepatic stellate cell activation and the development of liver fibrosis. Hepatology 67:2414-2429 |
| Savage, Thomas M; Shonts, Brittany A; Obradovic, Aleksandar et al. (2018) Early expansion of donor-specific Tregs in tolerant kidney transplant recipients. JCI Insight 3: |
| Wu, Wen-Hsuan; Tsai, Yi-Ting; Justus, Sally et al. (2018) CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology. Methods Mol Biol 1715:191-205 |
| Jauregui, Ruben; Park, Karen Sophia; Tsang, Stephen H (2018) Two-year progression analysis of RPE65 autosomal dominant retinitis pigmentosa. Ophthalmic Genet 39:544-549 |
| Ishida, Chiaki T; Zhang, Yiru; Bianchetti, Elena et al. (2018) Metabolic Reprogramming by Dual AKT/ERK Inhibition through Imipridones Elicits Unique Vulnerabilities in Glioblastoma. Clin Cancer Res 24:5392-5406 |
| Yen, Bonnie; Fortson, Katherine T; Rothman, Nyanza J et al. (2018) Clonal Bifurcation of Foxp3 Expression Visualized in Thymocytes and T Cells. Immunohorizons 2:119-128 |
| Renz, Bernhard W; Takahashi, Ryota; Tanaka, Takayuki et al. (2018) ?2 Adrenergic-Neurotrophin Feedforward Loop Promotes Pancreatic Cancer. Cancer Cell 33:75-90.e7 |
| Jin, Chun-Hui; Li, Yang; Xia, Jinxing et al. (2018) CXCR4 blockade improves leukemia eradication by allogeneic lymphocyte infusion. Am J Hematol 93:786-793 |
| Bakhoum, Mathieu F; Sengillo, Jesse D; Cui, Xuan et al. (2018) AUTOIMMUNE RETINOPATHY IN A PATIENT WITH A MISSENSE MUTATION IN PITPNM3. Retin Cases Brief Rep 12 Suppl 1:S72-S75 |
Showing the most recent 10 out of 331 publications
