Research at the Koch Institute combines a wide spectrum of innovative programs designed to understand tumor development and develop new treatments and diagnostic strategies. Many of the approaches taken, in disciplines ranging from molecular and cell biological to analysis of mouse models and nanotechnology, rely on imaging. In 2005, using non-renewable institutional funds, the Koch Institute established a Microscopy Core Facility that makes centralized, state-of-the art imaging equipment available to Center investigators and other NCI-funded investigators at MIT. CCSG funds are requested to provide continuing support for this Core. The Microscopy Core currently offers a wide range of imaging platforms including standard light and epifluoresence to digital deconvolution, spinning disk confocal microscopy, and systems for spectral Karyotyping (SKY) and laser capture microdissection. The Core is run by a highly qualified Core Manager, who has extensive expertise in a broad range of microscopy platforms and applications including electron microscopy and live cell/organotypic fluorescent 4D imaging. The Core Manager oversees all instrumentation, provides state-of-the-art training to Center members in both sample preparation and use of the Core's equipment and also conducts specific microscopy techniques for Kl users on a fee-for-service basis. She also collaborates with the Applied Therapeutics &Whole Animal Imaging Core Facility, providing technical support and user training for the whole-animal imaging instrumentation including luminescence/fluorescence detection and microCT applications. The Microscopy Core has proven invaluable to a large fraction of the Kl faculty by allowing them to execute microscopy-dependent projects that were previously impossible given the expense of acquiring and maintaining imaging equipment. In the upcoming grant period, the Kl will continue to add new equipment to both the Microscopy and Applied Therapeutics &Whole Animal Imaging Core Facilities to further expanding their imaging capabilities. This expansion, as well as high demand for existing services, requires addition of a technical assistant to the Microscopy Core Facility staff. User chargebacks provide partial support the Microscopy Core, but the operational costs are currently underwritten by Institutional funds that will expire in June 2009. Thus, the requested CCSG support is critical for the continued success of this essential Microscopy Core.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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Massachusetts Institute of Technology
United States
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Rothenberg, Daniel A; Taliaferro, J Matthew; Huber, Sabrina M et al. (2018) A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth. iScience 9:367-381
Kimmerling, Robert J; Prakadan, Sanjay M; Gupta, Alejandro J et al. (2018) Linking single-cell measurements of mass, growth rate, and gene expression. Genome Biol 19:207
Tang, Li; Zheng, Yiran; Melo, Mariane Bandeira et al. (2018) Enhancing T cell therapy through TCR-signaling-responsive nanoparticle drug delivery. Nat Biotechnol 36:707-716
Holec, Patrick V; Berleant, Joseph; Bathe, Mark et al. (2018) A Bayesian framework for high-throughput T cell receptor pairing. Bioinformatics :
Wong, Madeline Y; Doan, Ngoc Duc; DiChiara, Andrew S et al. (2018) A High-Throughput Assay for Collagen Secretion Suggests an Unanticipated Role for Hsp90 in Collagen Production. Biochemistry 57:2814-2827
Danai, Laura V; Babic, Ana; Rosenthal, Michael H et al. (2018) Altered exocrine function can drive adipose wasting in early pancreatic cancer. Nature 558:600-604
Dubbury, Sara J; Boutz, Paul L; Sharp, Phillip A (2018) CDK12 regulates DNA repair genes by suppressing intronic polyadenylation. Nature 564:141-145
Tokatlian, Talar; Kulp, Daniel W; Mutafyan, Andrew A et al. (2018) Enhancing Humoral Responses Against HIV Envelope Trimers via Nanoparticle Delivery with Stabilized Synthetic Liposomes. Sci Rep 8:16527
Crowell, Laura E; Lu, Amos E; Love, Kerry R et al. (2018) On-demand manufacturing of clinical-quality biopharmaceuticals. Nat Biotechnol :
Lo, Justin H; Hao, Liangliang; Muzumdar, Mandar D et al. (2018) iRGD-guided Tumor-penetrating Nanocomplexes for Therapeutic siRNA Delivery to Pancreatic Cancer. Mol Cancer Ther 17:2377-2388

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