The Flow and Image Cytometry Shared Resource (FICR) provides advanced flow cytometric and morphology service utilizing state-of-the-art technology and an extensive computer network that has been assembled into a user-friendly and cost-effective environment. The Resource is supervised by Paul K. Wallace, PhD, and Hans Minderman, PhD, and is staffed by an additional 10 technical personnel with responsibilities for operating and maintaining the equipment and assisting users with their flow cytometric and imaging experiments. The goals are to provide (i) multiparameter flow cytometry, encompassing analytical, sorting and Luminex services, (ii) Confocal, Live Cell and ImageStream imaging services, and (iii) instruction and technical support as required by investigators. As a focal point for many interdisciplinary activities throughout the cancer center, the Resource supports both basic research and clinical protocol services. The Resource's strategic plan emphasizes the provision of state-of-the-art technology and expertise in all facets of flow and image cytometry, and supporting the needs of CCSG Program members to enhance peer-reviewed funding and publications. The flow cytometry component of the Resource offers both clinical and basic research support services, which universally maintain a quality assurance program using guidelines from NY State and national accrediting agencies. The imaging component enables qualitative and quantitative image analysis of cells at the cellular and subcellular levels, time-kinetic multicolor image analysis and quantitative multispectral image analysis. The educational program component provides didactic lectures and hands-on experience with (i) isolation, preparation, and staining of all types of human and animal cells, (ii) instrument setup and acquisition, and (iii) data analysis. First priority for use is given to peer-review-funded CCSG members;second priority to non-peer-review-funded CCSG members;third priority to non-members and academic collaborators;and last priority to external users. During the reporting period, the Flow and Image Cytometry Shared Resource has served 75 members from 6 research programs, with 40% utilization by CCSG members with peer reviewed funding. The CCSG support provides 6% of the overall proposed budget.

Public Health Relevance

The ability to analyze the expression of molecules on the surface and within individual cells, and to sort those cells based on expression of those molecules, is an essential tool for many cancer investigators. The cancer research supported by this shared resource provides state-of-the-art facilities and outstanding expertise in flow cytometry and cell sorting.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Roswell Park Cancer Institute Corp
United States
Zip Code
Ciamporcero, Eric; Miles, Kiersten Marie; Adelaiye, Remi et al. (2015) Combination strategy targeting VEGF and HGF/c-met in human renal cell carcinoma models. Mol Cancer Ther 14:101-10
Ma, Yingyu; Hu, Qiang; Luo, Wei et al. (2015) 1?,25(OH)2D3 differentially regulates miRNA expression in human bladder cancer cells. J Steroid Biochem Mol Biol 148:166-71
Shen, Li; Sundstedt, Anette; Ciesielski, Michael et al. (2015) Tasquinimod modulates suppressive myeloid cells and enhances cancer immunotherapies in murine models. Cancer Immunol Res 3:136-48
Adelaiye, Remi; Ciamporcero, Eric; Miles, Kiersten Marie et al. (2015) Sunitinib dose escalation overcomes transient resistance in clear cell renal cell carcinoma and is associated with epigenetic modifications. Mol Cancer Ther 14:513-22
Ambrosone, Christine B; Zirpoli, Gary; Ruszczyk, Melanie et al. (2014) Parity and breastfeeding among African-American women: differential effects on breast cancer risk by estrogen receptor status in the Women's Circle of Health Study. Cancer Causes Control 25:259-65
Uekusa, Shota; Kawashima, Hiroyuki; Sugito, Kiminobu et al. (2014) Nr4a3, a possibile oncogenic factor for neuroblastoma associated with CpGi methylation within the third exon. Int J Oncol 44:1669-77
Rohrbach, Daniel J; Muffoletto, Daniel; Huihui, Jonathan et al. (2014) Preoperative mapping of nonmelanoma skin cancer using spatial frequency domain and ultrasound imaging. Acad Radiol 21:263-70
Röhm, Marc; Grimm, Melissa J; D'Auria, Anthony C et al. (2014) NADPH oxidase promotes neutrophil extracellular trap formation in pulmonary aspergillosis. Infect Immun 82:1766-77
Haller, Andrew C; Tan, Wei; Payne-Ondracek, Rochelle et al. (2014) High SPDEF may identify patients who will have a prolonged response to androgen deprivation therapy. Prostate 74:509-19
Ambrosone, Christine B; Young, Allyson C; Sucheston, Lara E et al. (2014) Genome-wide methylation patterns provide insight into differences in breast tumor biology between American women of African and European ancestry. Oncotarget 5:237-48

Showing the most recent 10 out of 517 publications