The OSUCCC Analytical Cytometry Shared Resource (ACSR) is an extensive, institutionally-supported shared service. This core provides one of the only means of rapidly and accurately analyzing multiple characteristics of biological particles while also being able to rapidly, accurately, and with high purity (>98%) sort out pure populations of cells of interest based on parameters designated by the investigator. Furthermore, this service provides OSUCCC members with the ability to obtain viable, sterile and pure populations of cells so that they may be individually cloned, can be assessed for immunological function, or can be examined for specific biochemical properties with minimal manipulations, compared to magnetic bead technologies. This shared resource has five primary goals: 1) Provide state of the art flow cytometry analysis and sorting on a fee-for-service basis;2) Provide individual training followed by 24-hour access to flow cytometry instrumentation for researchers who wish to conduct their own analysis;3) Develop and provide educational and training opportunities for new and experienced resource users as well as forums to introduce new instrumentation, technologies and reagents to OSUCCC investigators;4) Obtain and provide state-of-the-art equipment to support high quality cancer research for OSUCCC members;and 5) Introduce new, or pre-commercial, emerging technology to support high quality cancer research for OSUCCC members. The ACSR main facility is centrally located and has eight flow cytometry instruments, four of which are capable of sorting. Two flow cytometer analyzers are available for independent (24 hour access) and assisted analysis. In addition, commercial and prototype magnetic separation and analysis equipment is available. Five'of these instruments were purchased with institufional support of approximately $1,358,000 in the last four years. In order to meet the needs of heavy users and maintain adequate space and access, the ACSR has two satellite facilifies located in the James Cancer Hospital (JCH) and the OSU College of Veterinary Medicine (CVM). The CVM has three flow cytometers, one of which is equipped to safely sort virus infected cells. The ACSR Director is Jeffrey Chalmers, Ph.D. with a manager, Bryan McElwain, and two additional staff. The CVM satellite is managed by A. Nicole White and has an additional technician. In addition, this past year Mary Jo Burkhard, D.V.M., Ph.D. was recruited as a co-investigator in the ACSR focused on education and outreach. The ACSR continues to provide critical support to the investigators and scientific programs, including 14 clinical studies acfively using the services of the ACSR This past year, nearly 75% of the ACSR usage was from 63 CCSG peer-reviewed, funded OSUCCC investigators from all six programs who consumed over 4,300 hours of service, yet only 23.4% of the support came from the CCSG.
The ACSR provides instrumentation and technical operation/support for cell identification, characterization and cell separation to OSUCCC members and the University community. The ACSR, through exceptional institutional support and experienced leadership, is designed to provide affordable and high quality service in each of these areas, based on a cost-effective charge-back system. This ACSR provides critical support to OSUCCC scientific programs and clinical studies, while contributing outstanding technical expertise to high quality scientific cancer research.
|Bolyard, Chelsea; Meisen, W Hans; Banasavadi-Siddegowda, Yeshavanth et al. (2017) BAI1 Orchestrates Macrophage Inflammatory Response to HSV Infection-Implications for Oncolytic Viral Therapy. Clin Cancer Res 23:1809-1819|
|Schuh, Elizabeth M; Portela, Roberta; Gardner, Heather L et al. (2017) Safety and efficacy of targeted hyperthermia treatment utilizing gold nanorod therapy in spontaneous canine neoplasia. BMC Vet Res 13:294|
|Kumar, Bhavna; Yadav, Arti; Brown, Nicole V et al. (2017) Nuclear PRMT5, cyclin D1 and IL-6 are associated with poor outcome in oropharyngeal squamous cell carcinoma patients and is inversely associated with p16-status. Oncotarget 8:14847-14859|
|Miller, Cecelia R; Ruppert, Amy S; Fobare, Sydney et al. (2017) The long noncoding RNA, treRNA, decreases DNA damage and is associated with poor response to chemotherapy in chronic lymphocytic leukemia. Oncotarget 8:25942-25954|
|Pearlman, Rachel; Frankel, Wendy L; Swanson, Benjamin et al. (2017) Prevalence and Spectrum of Germline Cancer Susceptibility Gene Mutations Among Patients With Early-Onset Colorectal Cancer. JAMA Oncol 3:464-471|
|Farren, Matthew R; Hennessey, Rebecca C; Shakya, Reena et al. (2017) The Exportin-1 Inhibitor Selinexor Exerts Superior Antitumor Activity when Combined with T-Cell Checkpoint Inhibitors. Mol Cancer Ther 16:417-427|
|Teng, Kun-Yu; Han, Jianfeng; Zhang, Xiaoli et al. (2017) Blocking the CCL2-CCR2 Axis Using CCL2-Neutralizing Antibody Is an Effective Therapy for Hepatocellular Cancer in a Mouse Model. Mol Cancer Ther 16:312-322|
|Russell, Luke; Bolyard, Chelsea; Banasavadi-Siddegowda, Yeshavanth et al. (2017) Sex as a biological variable in response to temozolomide. Neuro Oncol 19:873-874|
|Terrazas, Cesar; de Dios Ruiz-Rosado, Juan; Amici, Stephanie A et al. (2017) Helminth-induced Ly6Chi monocyte-derived alternatively activated macrophages suppress experimental autoimmune encephalomyelitis. Sci Rep 7:40814|
|Saporito, Donika; Brock, Pamela; Hampel, Heather et al. (2017) Penetrance of a rare familial mutation predisposing to papillary thyroid cancer. Fam Cancer :|
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