The Flow Cytometry shared resource of the Virginia Commonwealth (VCU) Massey Cancer Center (MCC) was founded in 1976. Its instrumentation has been regularly upgraded through eight generations of flow cytometers to the five flow instruments currently in use, two multi-laser BD Aria II sorters, one of which is located in a Baker BioProtect Hood, three analyzers, a BD Canto II, a Coulter FC500 and a Coulter XL-MCL. Capabilities include up to nine color simultaneous detection and up to four simultaneous sorts per Aria II. The Flow Cytometry shared resource also has a Biacore T100 instrument for surface plasmon resonance (SPR) studies. The Director, Dr Daniel H. Conrad, has over 25 years of experience in flow cytometry and over 12 years of experience with SPR technology. Dr Conrad, whose office and laboratory are in close proximity to the instruments in the Kontos Medical Science Building (KMSB), meets regularly with core personnel and oversees planning for the new facility. The Resource Manager, Ms Julie Farnsworth, is highly trained in flow cytometry and has been with the Flow Cytometry shared resource for nine years. Previous to this, she worked for the Flow Cytometry core at the University of North Carolina (UNC) for two years. The resource provides a wide range of services, from routine fluorescence analysis to interactive custom design of innovative analysis and sorting protocols, addressing the specific needs of individual investigators. Dr Conrad closely follows the analyses performed by the core facility, insuring quality control, and advising facility users on alternative, often novel, approaches to experimental design and data analysis. Since the last review, the resource has upgraded facilities and instrumentation, moved to a new central location and added the capabilities for multiple high-speed and biohazardous sorting. This shared resource maintains a stable base of instrumentation, expertise and trained personnel and currently provides services and training for multiple users in 69 research groups from 21 departments at VCU. It has had a major impact on cancer research at the VCU MCC for 30 years.
Flow cytometry has important applications in basic, translational, and clinical cancer research. Flow cytometry is used as a tool to measure molecules both on and in individual cells. This capacity gives cancer researchers the ability to identify the differences between normal and cancerous cells, to quantify the immune response to cancer cells, and to measure the response of cancer cells to treatment.
|Ding, Boxiao; Parmigiani, Anita; Divakaruni, Ajit S et al. (2016) Sestrin2 is induced by glucose starvation via the unfolded protein response and protects cells from non-canonical necroptotic cell death. Sci Rep 6:22538|
|Terracina, Krista P; Graham, Laura J; Payne, Kyle K et al. (2016) DNA methyltransferase inhibition increases efficacy of adoptive cellular immunotherapy of murine breast cancer. Cancer Immunol Immunother 65:1061-73|
|Alotaibi, Moureq; Sharma, Khushboo; Saleh, Tareq et al. (2016) Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells. Radiat Res 185:229-45|
|Truchan, Hilary K; Cockburn, Chelsea L; Hebert, Kathryn S et al. (2016) The Pathogen-Occupied Vacuoles of Anaplasma phagocytophilum and Anaplasma marginale Interact with the Endoplasmic Reticulum. Front Cell Infect Microbiol 6:22|
|Menezes, M E; Das, S K; Minn, I et al. (2016) Detecting Tumor Metastases: The Road to Therapy Starts Here. Adv Cancer Res 132:1-44|
|Agarwal, Stuti; Bell, Catherine M; Taylor, Shirley M et al. (2016) p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb. Mol Cancer Res 14:66-77|
|Gewirtz, David A (2016) The Challenge of Developing Autophagy Inhibition as a Therapeutic Strategy. Cancer Res 76:5610-5614|
|Lafata, Jennifer Elston; Shay, L Aubree; Brown, Richard et al. (2016) Office-Based Tools and Primary Care Visit Communication, Length, and Preventive Service Delivery. Health Serv Res 51:728-45|
|Korwar, Sudha; Morris, Benjamin L; Parikh, Hardik I et al. (2016) Design, synthesis, and biological evaluation of substrate-competitive inhibitors of C-terminal Binding Protein (CtBP). Bioorg Med Chem 24:2707-15|
|Ge, Xiuchun; Shi, Xiaoli; Shi, Limei et al. (2016) Involvement of NADH Oxidase in Biofilm Formation in Streptococcus sanguinis. PLoS One 11:e0151142|
Showing the most recent 10 out of 413 publications