-Flow Cytometry (FLOW) Shared Resource The Flow Cytometry Shared Resource (FLOW SR) is a basic shared resource in the basic group that provides flow cytometry research support for Cancer Center members. The overall mission of the SR is to provide technical, educational assistance for individuals performing flow cytometry and to assist investigators in data interpretation and the planning of future experiments that involve flow cytometry. The SR provides multi-color flow cytometry analysis, high speed sorting (including biohazard sorting), education, consultation, application development, and data analysis. Since the 2010 review, use of the shared resource has increased 63%. To accommodate and permit this increase the SR has increased both hardware and staff. We added two analytical cytometers: a Stratedigm S1000EX for microparticle analysis, a 4-laser BD LSRFortessa, and two cell sorters: a BD FACSAria II SORP in BSL-2 containment and a FACSAria III for investigator operation. The FACSAria II, which is configured for BSL-2 sorting has been placed in a specially renovated BSL-2 space with enhanced precautions. We also upgraded the Beckman MoFlo XDP with two additional lasers. Education initiatives have expanded considerably, including user-operated cell sorting on the FACSAria III.
The Specific Aims focus on providing access and training on existing equipment, integrating new equipment, providing expert research staff and provided that continuing education to users on new experimental methods. We added a highly skilled Technology Manager position to accommodate the increased use and to provide additional services including expertise for the incoming Amnis ImageStreamX imaging flow cytometer. Center member usage accounted for 54% of the total SR usage and resulted in publications in journals such as JCI, JPET, and Blood. For 2015, the FLOW SR Facility requests $122,656 for personnel and maintenance expenses. CCSG funds are projected to be 19% of operating costs.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA016086-42
Application #
9391963
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
42
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Kang, SunAh; Fedoriw, Yuri; Brenneman, Ethan K et al. (2017) BAFF Induces Tertiary Lymphoid Structures and Positions T Cells within the Glomeruli during Lupus Nephritis. J Immunol 198:2602-2611
Ehe, Ben K; Lamson, David R; Tarpley, Michael et al. (2017) Identification of a DYRK1A-mediated phosphorylation site within the nuclear localization sequence of the hedgehog transcription factor GLI1. Biochem Biophys Res Commun 491:767-772
Conway, Kathleen; Edmiston, Sharon N; Parrish, Eloise et al. (2017) Breast tumor DNA methylation patterns associated with smoking in the Carolina Breast Cancer Study. Breast Cancer Res Treat 163:349-361
Williams, Lindsay A; Olshan, Andrew F; Hong, Chi-Chen et al. (2017) Alcohol Intake and Breast Cancer Risk in African American Women from the AMBER Consortium. Cancer Epidemiol Biomarkers Prev 26:787-794
Quach, Bryan; Furey, Terrence S (2017) DeFCoM: analysis and modeling of transcription factor binding sites using a motif-centric genomic footprinter. Bioinformatics 33:956-963
Wang, Sheng; Wacker, Daniel; Levit, Anat et al. (2017) D4 dopamine receptor high-resolution structures enable the discovery of selective agonists. Science 358:381-386
Westmoreland, Katherine D; Montgomery, Nathan D; Stanley, Christopher C et al. (2017) Plasma Epstein-Barr virus DNA for pediatric Burkitt lymphoma diagnosis, prognosis and response assessment in Malawi. Int J Cancer 140:2509-2516
Bower, Jacquelyn J; Vance, Leah D; Psioda, Matthew et al. (2017) Patterns of cell cycle checkpoint deregulation associated with intrinsic molecular subtypes of human breast cancer cells. NPJ Breast Cancer 3:9
Hanson, Laura C; Collichio, Frances; Bernard, Stephen A et al. (2017) Integrating Palliative and Oncology Care for Patients with Advanced Cancer: A Quality Improvement Intervention. J Palliat Med 20:1366-1371
Krishnaiah, Saikumari Y; Wu, Gang; Altman, Brian J et al. (2017) Clock Regulation of Metabolites Reveals Coupling between Transcription and Metabolism. Cell Metab 25:961-974.e4

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