9.1.5 FLOW CYTOMETRY SHARED SERVICE The Flow Cytometry Shared Service (FCSS) has served investigators of the Arizona Cancer Center (AZCC) for over 20 years. We offer AZCC investigators efficient and reliable flow cytometric services with high standards of quality control and productivity. We provide the knowledge and assistance necessary to design experiments, develop new assays, perform flow cytometric functions and interpret data. Our service supports the developrnent of new and innovative basic and clinicalstudies in cancer prevention, carcinogenesis, and cell and niolecular biology, and laboratory-based pre-clinical and translational drug development studies of new cancer therapies utilizing flow cytometric techniques. Flow cytometry instrumentation comprises a FACScan, FACSAria and LSR II, along wth associated software packages for data analysis and presentation. A number of methodologies are offered: cell sorting, cell cycle/ploidy analysis, multi^fluorochrome immunophenotyping, apoptosis/necrosis analysis, multicytokine detection in supernatants/plasma, intracellular antigen measurement, and intracelluar pH/calcium analysis. Personnel include the Director, Dr. Amanda Baker, and two expert cytometrists, one of whom also serves as facility manager. The FCSS has experienced continuous growth from 2003 thru 2008, both with regard to the service itself and investigator usage. In 2004 the FCSS completed a merger with the University Arizona Research Laboratories (ARL) Cytometry Core Service. By combining administration, expertise and equipment, a premier FCSS was created for our AZCC investigators. Usage activity by AZCC program investigators has increased three-fold with the number of AZCC members using the facility increasing approximately two-fold (from 20 in 2003 to 38 in 2008). During this time period, a total of 48 peer-reviewed publications have been produced by AZCC investigators using the FCSS. Overall, the FCSS operates efficiently and effectively for our AZCC investigators with continued growth and usage.

Public Health Relevance

The Flow Cytometry Shared Service offers an affordable resource for expert assistance in experimental design, execution, and analysis for Conducting studies utilizing the latest technology in flow cytometery.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023074-34
Application #
8377795
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
34
Fiscal Year
2012
Total Cost
$101,099
Indirect Cost
$34,367
Name
University of Arizona
Department
Type
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Dickinson, Sally E; Janda, Jaroslav; Criswell, Jane et al. (2016) Inhibition of Akt Enhances the Chemopreventive Effects of Topical Rapamycin in Mouse Skin. Cancer Prev Res (Phila) 9:215-24
Schissler, A Grant; Li, Qike; Chen, James L et al. (2016) Analysis of aggregated cell-cell statistical distances within pathways unveils therapeutic-resistance mechanisms in circulating tumor cells. Bioinformatics 32:i80-i89
Banerjee, Bhaskar; Shaheen, Nicholas J; Martinez, Jessica A et al. (2016) Clinical Study of Ursodeoxycholic Acid in Barrett's Esophagus Patients. Cancer Prev Res (Phila) 9:528-33
Pulko, Vesna; Davies, John S; Martinez, Carmine et al. (2016) Human memory T cells with a naive phenotype accumulate with aging and respond to persistent viruses. Nat Immunol 17:966-75
Warfel, Noel A; Sainz, Alva G; Song, Jin H et al. (2016) PIM Kinase Inhibitors Kill Hypoxic Tumor Cells by Reducing Nrf2 Signaling and Increasing Reactive Oxygen Species. Mol Cancer Ther 15:1637-47
Thomson, Cynthia A; Ho, Emily; Strom, Meghan B (2016) Chemopreventive properties of 3,3'-diindolylmethane in breast cancer: evidence from experimental and human studies. Nutr Rev 74:432-43
Tsikitis, Vassiliki L; Potter, Amiee; Mori, Motomi et al. (2016) MicroRNA Signatures of Colonic Polyps on Screening and Histology. Cancer Prev Res (Phila) 9:942-949
Hanke, Neale T; Garland, Linda L; Baker, Amanda F (2016) Carfilzomib combined with suberanilohydroxamic acid (SAHA) synergistically promotes endoplasmic reticulum stress in non-small cell lung cancer cell lines. J Cancer Res Clin Oncol 142:549-60
Kohler, Lindsay N; Garcia, David O; Harris, Robin B et al. (2016) Adherence to Diet and Physical Activity Cancer Prevention Guidelines and Cancer Outcomes: A Systematic Review. Cancer Epidemiol Biomarkers Prev 25:1018-28
Landowski, Terry H; Guntle, Gerald P; Zhao, Dezheng et al. (2016) Magnetic Resonance Imaging Identifies Differential Response to Pro-Oxidant Chemotherapy in a Xenograft Model. Transl Oncol 9:228-35

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