9.1.5 FLOW CYTOMETRY SHARED SERVICE The Flow Cytometry Shared Service (FCSS) has served investigators of the Arizona Cancer Center (AZCC) for over 20 years. We offer AZCC investigators efficient and reliable flow cytometric services with high standards of quality control and productivity. We provide the knowledge and assistance necessary to design experiments, develop new assays, perform flow cytometric functions and interpret data. Our service supports the developrnent of new and innovative basic and clinicalstudies in cancer prevention, carcinogenesis, and cell and niolecular biology, and laboratory-based pre-clinical and translational drug development studies of new cancer therapies utilizing flow cytometric techniques. Flow cytometry instrumentation comprises a FACScan, FACSAria and LSR II, along wth associated software packages for data analysis and presentation. A number of methodologies are offered: cell sorting, cell cycle/ploidy analysis, multi^fluorochrome immunophenotyping, apoptosis/necrosis analysis, multicytokine detection in supernatants/plasma, intracellular antigen measurement, and intracelluar pH/calcium analysis. Personnel include the Director, Dr. Amanda Baker, and two expert cytometrists, one of whom also serves as facility manager. The FCSS has experienced continuous growth from 2003 thru 2008, both with regard to the service itself and investigator usage. In 2004 the FCSS completed a merger with the University Arizona Research Laboratories (ARL) Cytometry Core Service. By combining administration, expertise and equipment, a premier FCSS was created for our AZCC investigators. Usage activity by AZCC program investigators has increased three-fold with the number of AZCC members using the facility increasing approximately two-fold (from 20 in 2003 to 38 in 2008). During this time period, a total of 48 peer-reviewed publications have been produced by AZCC investigators using the FCSS. Overall, the FCSS operates efficiently and effectively for our AZCC investigators with continued growth and usage.

Public Health Relevance

The Flow Cytometry Shared Service offers an affordable resource for expert assistance in experimental design, execution, and analysis for Conducting studies utilizing the latest technology in flow cytometery.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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University of Arizona
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Justiniano, Rebecca; Williams, Joshua D; Perer, Jessica et al. (2017) The B6 -vitamer Pyridoxal is a Sensitizer of UVA-induced Genotoxic Stress in Human Primary Keratinocytes and Reconstructed Epidermis. Photochem Photobiol 93:990-998
Li, Qike; Schissler, A Grant; Gardeux, Vincent et al. (2017) N-of-1-pathways MixEnrich: advancing precision medicine via single-subject analysis in discovering dynamic changes of transcriptomes. BMC Med Genomics 10:27
Harris, Lauren N; Bauer, Margaret R; Wiley, Joshua F et al. (2017) Chronic and episodic stress predict physical symptom bother following breast cancer diagnosis. J Behav Med 40:875-885
Uhrlaub, Jennifer L; Smithey, Megan J; Nikolich-┼Żugich, Janko (2017) Cutting Edge: The Aging Immune System Reveals the Biological Impact of Direct Antigen Presentation on CD8 T Cell Responses. J Immunol 199:403-407
Justiniano, Rebecca; Perer, Jessica; Hua, Anh et al. (2017) A Topical Zinc Ionophore Blocks Tumorigenic Progression in UV-exposed SKH-1 High-risk Mouse Skin. Photochem Photobiol 93:1472-1482
Einspahr, Janine G; Curiel-Lewandrowski, Clara; Calvert, Valerie S et al. (2017) Protein activation mapping of human sun-protected epidermis after an acute dose of erythemic solar simulated light. NPJ Precis Oncol 1:
Sells, Earlphia; Pandey, Ritu; Chen, Hwudaurw et al. (2017) Specific microRNA-mRNA Regulatory Network of Colon Cancer Invasion Mediated by Tissue Kallikrein-Related Peptidase 6. Neoplasia 19:396-411
Polley, D J; Mihara, K; Ramachandran, R et al. (2017) Cockroach allergen serine proteinases: Isolation, sequencing and signalling via proteinase-activated receptor-2. Clin Exp Allergy 47:946-960
Bungard, Dixie; Copple, Jacob S; Yan, Jing et al. (2017) Foldability of a Natural De Novo Evolved Protein. Structure 25:1687-1696.e4
Das, Lipsa; Anderson, Todd A; Gard, Jaime M C et al. (2017) Characterization of Laminin Binding Integrin Internalization in Prostate Cancer Cells. J Cell Biochem 118:1038-1049

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