Abstract

Flow Cytometry The Flow Cytometry Shared Resource has been an integral component of the UCSD Cancer Center since 1990. It exists to provide peer-review, funded investigators from all Divisions of the Cancer Center with ready access to high-speed analysis and sorting of dissociated cell populations from clinical samples, animal experiments and cell culture studies. The services available from this Resource are as follows: Analytical Flow Cytometry: Utilizes a FACSCalibur? flow cytometer and a newly purchased FACSAria? flow cytometer. The FACSCalibur? is a bench-top flow cytometer that can be used for analysis of up to fourcolors of fluorescence. The FACSAria? is a three-laser flow cytometer for analyses capable of up to eleven colors of fluorescence, along with forward and side-anglejight scatter. The FACSAria? utilizes FACSDiva? software, which has many advanced features, such as biexponential scaling, adaptive gating, and automated fluorescence spectral compensation. The FACSCalibur? flow cytometer computer system uses CELLQuest? software for list-mode data recording and analysis. These services allow investigators to analyze cell populations for cell cycle status, surface and cytoplasmic antigen phenotype, apoptosis, or expression of a transgene (e.g. following transduction with green fluorescent protein [GFP]).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA023100-27S6
Application #
8468770
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
2014-04-30
Budget Start
2011-05-01
Budget End
2013-04-30
Support Year
27
Fiscal Year
2012
Total Cost
$217,617
Indirect Cost
$74,529

  Institution

Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Singh, Siddharth; Loomba, Rohit (2018) Role of two-dimensional shear wave elastography in the assessment of chronic liver diseases. Hepatology 67:13-15
Hartman, Sheri J; Nelson, Sandahl H; Myers, Emily et al. (2018) Randomized controlled trial of increasing physical activity on objectively measured and self-reported cognitive functioning among breast cancer survivors: The memory & motion study. Cancer 124:192-202
Hoffmann, Hanne M; Gong, Ping; Tamrazian, Anika et al. (2018) Transcriptional interaction between cFOS and the homeodomain-binding transcription factor VAX1 on the GnRH promoter controls Gnrh1 expression levels in a GnRH neuron maturation specific manner. Mol Cell Endocrinol 461:143-154
Liu, Xuxiang; Cao, Minghui; Palomares, Melanie et al. (2018) Metastatic breast cancer cells overexpress and secrete miR-218 to regulate type I collagen deposition by osteoblasts. Breast Cancer Res 20:127
Huang, Justin K; Carlin, Daniel E; Yu, Michael Ku et al. (2018) Systematic Evaluation of Molecular Networks for Discovery of Disease Genes. Cell Syst 6:484-495.e5
Kalyanaraman, Hema; Schwaerzer, Gerburg; Ramdani, Ghania et al. (2018) Protein Kinase G Activation Reverses Oxidative Stress and Restores Osteoblast Function and Bone Formation in Male Mice With Type 1 Diabetes. Diabetes 67:607-623
Hartman, Sheri J; Marinac, Catherine R; Cadmus-Bertram, Lisa et al. (2018) Sedentary Behaviors and Biomarkers Among Breast Cancer Survivors. J Phys Act Health 15:1-6
Wu, Yan; Tamayo, Pablo; Zhang, Kun (2018) Visualizing and Interpreting Single-Cell Gene Expression Datasets with Similarity Weighted Nonnegative Embedding. Cell Syst 7:656-666.e4
Dow, Michelle; Pyke, Rachel M; Tsui, Brian Y et al. (2018) Integrative genomic analysis of mouse and human hepatocellular carcinoma. Proc Natl Acad Sci U S A 115:E9879-E9888
Que, Xuchu; Hung, Ming-Yow; Yeang, Calvin et al. (2018) Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. Nature 558:301-306

Showing the most recent 10 out of 862 publications